SwePub - search: WFRF:(Ekwall Karl)

Enumeration	Reference	Cover	Find
1.	Olsson, T G S, et al. (author) Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast 1999 In: Current Genetics. - : Springer Science and Business Media LLC. - 0172-8083 .- 1432-0983. ; 35:2, s. 82-87 Journal article (peer-reviewed)abstract We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric. i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h(90) cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.

Olsson, T G S, et al. (author)
Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast
1999
In: Current Genetics. - : Springer Science and Business Media LLC. - 0172-8083 .- 1432-0983. ; 35:2, s. 82-87
Journal article (peer-reviewed)abstract
- We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric. i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h(90) cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.

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