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- Pollak, Joanna, et al.
(author)
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Production of Cystatin C Wild Type and Stabilized Mutants
- 2010
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In: EJIFCC. - 1650-3414. ; 20:4, s. 70-166
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Journal article (peer-reviewed)abstract
- Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.
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