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  • Uzun, K., et al. (author)
  • Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles
  • 2010
  • In: Journal of nanoparticle research. - : Springer Science and Business Media LLC. - 1388-0764 .- 1572-896X. ; 12:8, s. 3057-3067
  • Journal article (peer-reviewed)abstract
    • In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Baykal, A. (1)
Toprak, Muhammet (1)
Sozeri, H. (1)
Senel, M. (1)
Uzun, K. (1)
Cevik, E. (1)
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Abasiyanik, M. F. (1)
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University
Royal Institute of Technology (1)
Language
English (1)
Year
2010 (1)

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