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  • Nissan, Tracy, et al. (author)
  • Analyzing P-bodies in Saccharomyces cerevisiae
  • 2008
  • In: Methods in Enzymology. - 0076-6879 .- 1557-7988. ; 448, s. 507-520
  • Journal article (peer-reviewed)abstract
    • Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Nissan, Tracy (1)
Parker, Roy (1)
University
Umeå University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Year
2008 (1)

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