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  • Sundström, S, et al. (author)
  • Growth-state independent induction of specific proteins in Swiss 3T3 cells by interferon.
  • 1983
  • In: Journal of interferon research. - 0197-8357. ; 3:2, s. 223-9
  • Journal article (peer-reviewed)abstract
    • The early effects of interferon (IFN) on the pattern of protein synthesis in Swiss 3T3 mouse fibroblasts were investigated by two-dimensional gel electrophoresis of 35S-methionine labeled cell extracts. IFN induced within 4 h the synthesis of three proteins with molecular weights of 48, 49, and 50 kD. An increase in the rate of synthesis of a 31 kD protein was also found. These changes were observed after IFN treatment of exponentially growing cells, density-inhibited cells, serum-starved cells, and serum-stimulated quiescent cells. The same protein patterns were obtained from cells treated with IFN-alpha or IFN-beta. The IFN response was blocked by the addition of actinomycin D, implying de novo transcription of the corresponding species of messenger RNA.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Lundgren, E. (1)
Ny, Tor (1)
Sundström, S (1)
University
Umeå University (1)
Language
English (1)
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