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  • McQuaw, Carolyn M, et al. (author)
  • Localization of Sphingomyelin in Cholesterol Domains by Imaging Mass Spectrometry
  • 2007
  • In: Langmuir. - 0743-7463 .- 1520-5827. ; 23:10, s. 5645-5650
  • Journal article (peer-reviewed)abstract
    • The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete, and there is a small amount of both phospholipids distributed throughout.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Ewing, Andrew G, 195 ... (1)
Winograd, Nicholas (1)
McQuaw, Carolyn M (1)
Zheng, Leiliang (1)
University
University of Gothenburg (1)
Chalmers University of Technology (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Year
2007 (1)

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