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  • Urbansky, Anke, et al. (author)
  • Label-free acoustophoretic enrichment of mononuclear cells from blood
  • 2017
  • In: MicroTAS 2017 : Savannah, Georgia, USA - Savannah, Georgia, USA. - 1556-5904. - 9780692941836 ; 2017, s. 1309-1310
  • Conference paper (peer-reviewed)abstract
    • We are reporting an efficient, label-free and continuous separation of mononuclear cells (MNC), from blood using microchip acoustophoresis. In standard PBS buffer, MNCs and red blood cells (RBC) display overlapping acoustophoretic mobilities which compromise separation of these cell types from each other. In this paper we capitalize on the fact that MNC and RBC display different acoustophysical properties. By optimizing the buffer conditions and thereby changing the acoustic contrast factor, and hence the acoustophoretic mobility of the cells, a 2800-fold enrichment of MNCs vs. RBCs with MNC recoveries up to 88% was accomplished.
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  • Result 1-1 of 1
Type of publication
conference paper (1)
Type of content
peer-reviewed (1)
Author/Editor
Laurell, Thomas (1)
Scheding, Stefan (1)
Ohlsson, Pelle (1)
Urbansky, Anke (1)
Lenshof, Andreas (1)
University
Lund University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Medical and Health Sciences (1)
Year
2017 (1)

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