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  • Torsson, Emeli, et al. (author)
  • Field-Adapted Full Genome Sequencing of Peste-Des-Petits-Ruminants Virus Using Nanopore Sequencing
  • 2020
  • In: Frontiers in Veterinary Science. - : Frontiers Media SA. - 2297-1769. ; 7
  • Journal article (peer-reviewed)abstract
    • Peste-des-petits-ruminants virus (PPRV) is currently the focus of a control and eradication program. Full genome sequencing has the opportunity to become a powerful tool in the eradication program by improving molecular epidemiology and the study of viral evolution. PPRV is prevalent in many resource-constrained areas, with long distances to laboratory facilities, which can lack the correct equipment for high-throughput sequencing. Here we present a protocol for near full or full genome sequencing of PPRV. The use of a portable miniPCR and MinION brings the laboratory to the field and in addition makes the production of a full genome possible within 24 h of sampling. The protocol has been successfully used on virus isolates from cell cultures and field isolates from tissue samples of naturally infected goats.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Berg, Mikael (1)
Karlsson Lindsjö, Os ... (1)
Johansson Wensman, J ... (1)
Torsson, Emeli (1)
University
Swedish University of Agricultural Sciences (1)
Language
English (1)
Research subject (UKÄ/SCB)
Agricultural Sciences (1)
Year
2020 (1)

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