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  • Grey, Carl, et al. (author)
  • Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
  • 2009
  • In: Journal of chromatography. B. - : Elsevier BV. - 1570-0232 .- 1873-376X. ; 877:20-21, s. 1827-1832
  • Journal article (peer-reviewed)abstract
    • In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. 
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  • Result 1-1 of 1
Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Grey, Carl (1)
Jacobsson, Sven P (1)
Edebrink, Per (1)
Krook, Maria (1)
University
Stockholm University (1)
Lund University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Engineering and Technology (1)
Year
2009 (1)

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