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  • Cruz, Javier, 1990-, et al. (author)
  • High pressure inertial focusing for separating and concentrating bacteria at high throughput
  • 2017
  • In: Journal of Micromechanics and Microengineering. - : IOP PUBLISHING LTD. - 0960-1317 .- 1361-6439. ; 27:8
  • Journal article (peer-reviewed)abstract
    • Inertial focusing is a promising microfluidic technology for concentration and separation of particles by size. However, there is a strong correlation of increased pressure with decreased particle size. Theory and experimental results for larger particles were used to scale down the phenomenon and find the conditions that focus 1 mu m particles. High pressure experiments in robust glass chips were used to demonstrate the alignment. We show how the technique works for 1 mu m spherical polystyrene particles and for Escherichia coli, not being harmful for the bacteria at 50 mu l min(-1). The potential to focus bacteria, simplicity of use and high throughput make this technology interesting for healthcare applications, where concentration and purification of a sample may be required as an initial step.
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