1. |
- Cruz, Javier, 1990-, et al.
(author)
-
High pressure inertial focusing for separating and concentrating bacteria at high throughput
- 2017
-
In: Journal of Micromechanics and Microengineering. - : IOP PUBLISHING LTD. - 0960-1317 .- 1361-6439. ; 27:8
-
Journal article (peer-reviewed)abstract
- Inertial focusing is a promising microfluidic technology for concentration and separation of particles by size. However, there is a strong correlation of increased pressure with decreased particle size. Theory and experimental results for larger particles were used to scale down the phenomenon and find the conditions that focus 1 mu m particles. High pressure experiments in robust glass chips were used to demonstrate the alignment. We show how the technique works for 1 mu m spherical polystyrene particles and for Escherichia coli, not being harmful for the bacteria at 50 mu l min(-1). The potential to focus bacteria, simplicity of use and high throughput make this technology interesting for healthcare applications, where concentration and purification of a sample may be required as an initial step.
|
|