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1.	Nogly, P., et al. (author) Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography 2016 In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7 Journal article (peer-reviewed)abstract Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 angstrom resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

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Type of publication: journal article (1)

Type of content: peer-reviewed (1)

Author/Editor: Zhao, Y. (1); Spence, J (1); Frank, M. (1); Kubo, M (1); Kimura, T (1); Neutze, Richard, 196 ... (1); show more...; Dods, Robert, 1989 (1); Båth, Petra, 1988 (1); Chapman, H. N. (1); Iwata, S (1); Barty, A. (1); Boutet, S. (1); dePonte, D. (1); Williams, G. J. (1); Conrad, C (1); Milathianaki, D. (1); James, D. (1); White, T. A. (1); Berntsen, Peter, 197 ... (1); Hunter, M. S. (1); Weierstall, U. (1); Fromme, P. (1); Harimoorthy, Rajiv (1); Milne, C. (1); Nelson, G. (1); Nango, E. (1); Standfuss, J. (1); Sala, L (1); Beyerlein, K. R. (1); Bean, R. (1); Gati, C. (1); Nogly, P. (1); Schertler, G. (1); Panneels, V. (1); Li, C. F. (1); Abela, R. (1); Wu, W. T. (1); Coe, J. (1); Rheinberger, J. (1); Koglin, J. E. (1); show less...

University: University of Gothenburg (1); Chalmers University of Technology (1)

Language: English (1)

Research subject (UKÄ/SCB): Natural sciences (1)

Year: 2016 (1)

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