SwePub
Sök i SwePub databas

  Extended search

Träfflista för sökning "(LAR1:mau) srt2:(1985-1989)"

Search: (LAR1:mau) > (1985-1989)

  • Result 11-12 of 12
Sort/group result
   
EnumerationReferenceCoverFind
11.
  •  
12.
  • Sundqvist, Christer, et al. (author)
  • Spectral properties and chromophore rotation of phytochrome bound to substituted Sepharose
  • 1986
  • In: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 66, s. 185-190
  • Journal article (peer-reviewed)abstract
    • Brushite purified phytochrome from Avena sativa L. cv. Sol II was bound to phenyl Sepharose, octyl Sepharose, CNBr-activated Sepharose and to anti-phytochrome immunoglobulins immobilized on Sepharose. The spectral properties of phytochrome bound to anti-phytochrome immunoglobulins and to phenyl Sepharose were similar to phytochrome in solution. Phytochrome bound to CNBr-activated Sepharose or to octyl Sepharose showed reduced Pfr formation after red irradiation. The reversal to Pr with far-red light was only partial but a further increase at 667 nm took place slowly in the dark. A peak at 657 nm was seen in the difference spectrum between CNBr-activated Sepharose-bound phytochrome kept in darkness and the identical sample immediately after a far-red irradiation. The change in linear dichroism at 660 nm and 730 nm, induced by plane polarized red or far-red light, was measured. It was computed that the long-wavelength transition moment of phytochrome had an average rotation angle of 31.5° or 180°–31.5°. The substrate used for immobilization had a limited effect on the rotation angle. Phytochrome immobilized on CNBr-activated Sepharose gave an angle of 27.8° and phytochrome immobilized on phenyl Sepharose gave an angle of 32.6°.
  •  
Skapa referenser, mejla, bekava och länka
  • Result 11-12 of 12

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view