SwePub
Sök i SwePub databas

  Extended search

Träfflista för sökning "L773:0168 1656 OR L773:1873 4863 srt2:(1995-1999)"

Search: L773:0168 1656 OR L773:1873 4863 > (1995-1999)

  • Result 11-13 of 13
Sort/group result
   
EnumerationReferenceCoverFind
11.
  • Ademark, Pia, et al. (author)
  • Softwood hemicellulose-degrading enzymes from Aspergillus niger: Purification and properties of a β-mannanase
  • 1998
  • In: Journal of Biotechnology. - 1873-4863. ; 63:3, s. 199-210
  • Journal article (peer-reviewed)abstract
    • The enzymes needed for galactomannan hydrolysis, i.e. β-mannanase, α-galactosidase and β-mannosidase, were produced by the filamentous fungus Aspergillus niger. The β-mannanase was purified to electrophoretic homogeneity in three steps using ammonium sulfate precipitation, anion-exchange chromatography and gel filtration. The purified enzyme had an isoelectric point of 3.7 and a molecular mass of 40 kDa. Ivory nut mannan was degraded mainly to mannobiose and mannotriose when incubated with the β-mannanase. Analysis by 1H NMR spectroscopy during hydrolysis of mannopentaose showed that the enzyme acts by the retaining mechanism. The N-terminus of the purified A. niger β-mannanase was sequenced by Edman degradation, and comparison with Aspergillus aculeatus β-mannanase indicated high identity. The enzyme most probably lacks a cellulose binding domain since it was unable to adsorb on cellulose.
  •  
12.
  •  
13.
  •  
Skapa referenser, mejla, bekava och länka
  • Result 11-13 of 13

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view