| 11. |
- Chatterjee, S., et al.
(author)
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Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers
- 2019
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In: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 19:21-22
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Journal article (peer-reviewed)abstract
- While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics‐centric study investigates a possible link between protein paucimannosylation, an under‐studied class of human N‐glycosylation [Man1‐3GlcNAc2Fuc0‐1], and cancer. The paucimannosidic glycans (PMGs) of 34 cancer cell lines and 133 tissue samples spanning 11 cancer types and matching non‐cancerous specimens are profiled from 467 published and unpublished PGC‐LC‐MS/MS N‐glycome datasets collected over a decade. PMGs, particularly Man2‐3GlcNAc2Fuc1, are prominent features of 29 cancer cell lines, but the PMG level varies dramatically across and within the cancer types (1.0–50.2%). Analyses of paired (tumor/non‐tumor) and stage‐stratified tissues demonstrate that PMGs are significantly enriched in tumor tissues from several cancer types including liver cancer (p = 0.0033) and colorectal cancer (p = 0.0017) and is elevated as a result of prostate cancer and chronic lymphocytic leukaemia progression (p < 0.05). Surface expression of paucimannosidic epitopes is demonstrated on human glioblastoma cells using immunofluorescence while biosynthetic involvement of N‐acetyl‐β‐hexosaminidase is indicated by quantitative proteomics. This intriguing association between protein paucimannosylation and human cancers warrants further exploration to detail the biosynthesis, cellular location(s), protein carriers, and functions of paucimannosylation in tumorigenesis and metastasis.
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| 12. |
- John, P. R., et al.
(author)
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In-beam gamma-ray spectroscopy of the neutron-rich platinum isotope Pt-200 toward the N=126 shell gap
- 2017
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In: Physical Review C. - : AMER PHYSICAL SOC. - 2469-9985 .- 2469-9993. ; 95:6
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Journal article (peer-reviewed)abstract
- The neutron-rich nucleus Pt-200 is investigated via in-beam gamma-ray spectroscopy to study the shape evolution in the neutron-rich platinum isotopes towards the N = 126 shell closure. The two-neutron transfer reaction Pt-198(Se-82, Se-80)Pt-200 is used to populate excited states of Pt-200. The Advanced Gamma Ray Tracking Array (AGATA) demonstrator coupled with the PRISMA spectrometer detects gamma rays coincident with the Se-80 recoils, the binary partner of Pt-200. The binary partner method is applied to extract the gamma-ray transitions and build the level scheme of Pt-200. The level at 1884 keV reported by Yates et al. [S. W. Yates, E. M. Baum, E. A. Henry, L. G. Mann, N. Roy, A. Aprahamian, R. A. Meyer, and R. Estep, Phys. Rev. C 37, 1889 (1988)] was confirmed to be at 1882.1 keV and assigned as the (6(1)(+)) state. An additional gamma ray was found and it presumably deexcites the (8(1)(+)) state. The results are compared with state-of-the-art beyond mean-field calculations, performed for the even-even Pt190-204 isotopes, revealing that Pt-200 marks the transition from the gamma-unstable behavior of lighter Pt nuclei towards a more spherical one when approaching the N = 126 shell closure.
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| 13. |
- John, P. R., et al.
(author)
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Shape evolution in the neutron-rich osmium isotopes : Prompt gamma-ray spectroscopy of Os-196
- 2014
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In: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 90:2, s. 021301-
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Journal article (peer-reviewed)abstract
- The shape transition in the neutron-rich Os isotopes is studied by investigating the neutron-rich Os-196 nucleus through in-beam gamma-ray spectroscopy using a two-proton transfer reaction from a Pt-198 target to a Se-82 beam. The beam-like recoils were detected and identified with the large-acceptance magnetic spectrometer PRISMA, and the coincident gamma rays were measured with the advanced gamma tracking array (AGATA) demonstrator. The de-excitation of the low-lying levels of the yrast-band of Os-196 were identified for the first time. The results are compared with state-of-the-art beyond-mean-field calculations, performed for the even-even Os188-198 isotopes. The new results suggest a smooth transition in the Os isotopes from a more axial rotational behavior towards predominately vibrational nuclei through triaxial configurations. An almost perfect gamma-unstable/triaxial rotor yrast band is predicted for Os-196 which is in agreement with the experimentally measured excited states.
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| 14. |
- Tillmann, J., et al.
(author)
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Evaluating Sex and Age Differences in ADI-R and ADOS Scores in a Large European Multi-site Sample of Individuals with Autism Spectrum Disorder
- 2018
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In: Journal of Autism and Developmental Disorders. - : Springer Science and Business Media LLC. - 0162-3257 .- 1573-3432. ; 48:7, s. 2490-2505
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Journal article (peer-reviewed)abstract
- Research on sex-related differences in Autism Spectrum Disorder (ASD) has been impeded by small samples. We pooled 28 datasets from 18 sites across nine European countries to examine sex differences in the ASD phenotype on the ADI-R (376 females, 1763 males) and ADOS (233 females, 1187 males). On the ADI-R, early childhood restricted and repetitive behaviours were lower in females than males, alongside comparable levels of social interaction and communication difficulties in females and males. Current ADI-R and ADOS scores showed no sex differences for ASD severity. There were lower socio-communicative symptoms in older compared to younger individuals. This large European ASD sample adds to the literature on sex and age variations of ASD symptomatology.
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