| 11. |
- Mestdagh, P., et al.
(author)
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An integrative genomics screen uncovers ncRNA T-UCR functions in neuroblastoma tumours
- 2010
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In: Oncogene. - : Springer Science and Business Media LLC. - 1476-5594 .- 0950-9232. ; 29:24, s. 3583-3592
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Journal article (peer-reviewed)abstract
- Different classes of non-coding RNAs, including microRNAs, have recently been implicated in the process of tumourigenesis. In this study, we examined the expression and putative functions of a novel class of non-coding RNAs known as transcribed ultraconserved regions (T-UCRs) in neuroblastoma. Genome-wide expression pro. ling revealed correlations between specific T-UCR expression levels and important clinicogenetic parameters such as MYCN amplification status. A functional genomics approach based on the integration of multi-level transcriptome data was adapted to gain insights into T-UCR functions. Assignments of T-UCRs to cellular processes such as TP53 response, differentiation and proliferation were verified using various cellular model systems. For the first time, our results de. ne a T-UCR expression landscape in neuroblastoma and suggest widespread T-UCR involvement in diverse cellular processes that are deregulated in the process of tumourigenesis. Oncogene (2010) 29, 3583-3592; doi:10.1038/onc.2010.106; published online 12 April 2010
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| 12. |
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| 13. |
- Schleiermacher, G., et al.
(author)
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Emergence of New ALK Mutations at Relapse of Neuroblastoma
- 2014
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In: Journal of Clinical Oncology. - : American Society of Clinical Oncology (ASCO). - 0732-183X .- 1527-7755. ; 32:25, s. 2727-2734
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Journal article (peer-reviewed)abstract
- Purpose In neuroblastoma, the ALK receptor tyrosine kinase is activated by point mutations. We investigated the potential role of ALK mutations in neuroblastoma clonal evolution. We analyzed ALK mutations in 54 paired diagnosis-relapse neuroblastoma samples using Sanger sequencing. When an ALK mutation was observed in one paired sample, a minor mutated component in the other sample was searched for by more than 100,000 x deep sequencing of the relevant hotspot, with a sensitivity of 0.17%. All nine ALK-mutated cases at diagnosis demonstrated the same mutation at relapse, in one case in only one of several relapse nodules. In five additional cases, the mutation seemed to be relapse specific, four of which were investigated by deep sequencing. In two cases, no mutation evidence was observed at diagnosis. In one case, the mutation was present at a subclonal level (0.798%) at diagnosis, whereas in another case, two different mutations resulting in identical amino acid changes were detected, one only at diagnosis and the other only at relapse. Further evidence of clonal evolution of ALK-mutated cells was provided by establishment of a fully ALK-mutated cell line from a primary sample with an ALK-mutated cell population at subclonal level (6.6%). In neuroblastoma, subclonal ALK mutations can be present at diagnosis with subsequent clonal expansion at relapse. Given the potential of ALK-targeted therapy, the significant spatiotemporal variation of ALK mutations is of utmost importance, highlighting the potential of deep sequencing for detection of subclonal mutations with a sensitivity 100-fold that of Sanger sequencing and the importance of serial samplings for therapeutic decisions.
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| 14. |
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| 15. |
- Bailo, Daniele, et al.
(author)
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The ENVRI Catalogue of Services : A Road-Test for Environmental Research Infrastructure Metadata Harmonisation and Interoperability
- 2024
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In: Metadata and Semantic Research - 17th Research Conference, MTSR 2023, Revised Selected Papers. - 1865-0929 .- 1865-0937. - 9783031659904 - 9783031659898 ; 2048 CCIS, s. 267-277
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Conference paper (peer-reviewed)abstract
- The European Environmental Research Infrastructures (ENVRI) community, in its pursuit of enabling seamless and FAIR (Findable, Accessible, Interoperable, and Reusable) access to environmental research data and services, has realised the ENVRI Catalogue of Services. This paper provides an in-depth look into the creation of this tool, highlighting the process, the challenges faced, and the scientific novelty it brings to the field of environmental research. From the conceptualisation of its metadata schema through the semantic enrichment processes involved, to filling it with content, we examine the steps taken to ensure that the Catalogue meets the demands of a diverse set of Research Infrastructures and we report the lesson learnt in an endeavour which involved 5 international Organizations and 6 Research Infrastructures in providing access to more than 70 multidisciplinary services from the Environmental domain.
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| 16. |
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| 17. |
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| 18. |
- Krebs, Frederik C, et al.
(author)
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A round robin study of flexible large-area roll-to-roll processed polymer solar cell modules
- 2009
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In: SOLAR ENERGY MATERIALS AND SOLAR CELLS. - : Elsevier BV. - 0927-0248. ; 93:11, s. 1968-1977
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Journal article (peer-reviewed)abstract
- A round robin for the performance of roll-to-roll coated flexible large-area polymer solar-cell modules involving 18 different laboratories in Northern America, Europe and Middle East is presented. The study involved the performance measurement of the devices at one location (Riso DTU) followed by transportation to a participating laboratory for performance measurement and return to the starting location (Riso DTU) for re-measurement of the performance. It was found possible to package polymer solar-cell modules using a flexible plastic barrier material in such a manner that degradation of the devices played a relatively small role in the experiment that has taken place over 4 months. The method of transportation followed both air-mail and surface-mail paths.
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