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Sökning: (L773:1087 0156 OR L773:1546 1696) srt2:(2015-2019) > (2015)

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  • Boekel, Jorrit, et al. (författare)
  • Multi-omic data analysis using Galaxy
  • 2015
  • Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 33:2, s. 137-9
  • Tidskriftsartikel (refereegranskat)
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  • Bosley, Katrine S, et al. (författare)
  • CRISPR germline engineering : the community speaks
  • 2015
  • Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 33:5, s. 478-486
  • Tidskriftsartikel (refereegranskat)
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  • Faridani, OR, et al. (författare)
  • Putting cells in their place
  • 2015
  • Ingår i: Nature biotechnology. - : Springer Science and Business Media LLC. - 1546-1696 .- 1087-0156. ; 33:5, s. 490-491
  • Tidskriftsartikel (refereegranskat)
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  • Wittrup, Anders, et al. (författare)
  • Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown.
  • 2015
  • Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1546-1696 .- 1087-0156. ; 33:8, s. 870-870
  • Tidskriftsartikel (refereegranskat)abstract
    • A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient process, we studied the uptake and cytosolic release of siRNAs, formulated in lipoplexes or lipid nanoparticles, by live-cell imaging and correlated it with knockdown of a target GFP reporter. siRNA release occurred invariably from maturing endosomes within ∼5-15 min of endocytosis. Cytosolic galectins immediately recognized the damaged endosome and targeted it for autophagy. However, inhibiting autophagy did not enhance cytosolic siRNA release. Gene knockdown occurred within a few hours of release and required <2,000 copies of cytosolic siRNAs. The ability to detect cytosolic release of siRNAs and understand how it is regulated will facilitate the development of rational strategies for improving the cytosolic delivery of candidate drugs.
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  • Xiao, L., et al. (författare)
  • A catalog of the mouse gut metagenome
  • 2015
  • Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 33:10
  • Tidskriftsartikel (refereegranskat)abstract
    • We established a catalog of the mouse gut metagenome comprising similar to 2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a low-fat or high-fat diet. Similar to the human gut microbiome, >99% of the cataloged genes are bacterial. We identified 541 metagenomic species and defined a core set of 26 metagenomic species found in 95% of the mice. The mouse gut microbiome is functionally similar to its human counterpart, with 95.2% of its Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologous groups in common. However, only 4.0% of the mouse gut microbial genes were shared (95% identity, 90% coverage) with those of the human gut microbiome. This catalog provides a useful reference for future studies.
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