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Träfflista för sökning "(WAKA:ref) lar1:(uu) srt2:(1980-1994)"

Search: (WAKA:ref) lar1:(uu) > (1980-1994)

  • Result 1-10 of 3824
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1.
  • Abdulkarim, Farhad, et al. (author)
  • Missense substitutions lethal to essential functions of EF-Tu
  • 1991
  • In: Biochimie. - : Elsevier BV. - 0300-9084 .- 1638-6183. ; 73:12, s. 1457-1464
  • Journal article (peer-reviewed)abstract
    • We have used a simple selection and screening method to isolate function defective mutants of EF-Tu. From 28 mutants tested, 12 different missense substitutions, individually lethal to some essential function of EF-Tu, were identified by sequencing. In addition we found a new non-lethal missense mutation. The frequency of isolation of unique mutations suggests that this method can be used to easily isolate many more. The lethal mutations occur in all three structural domains of EF-Tu, but most are in domain II. We aim to use these mutants to define functional domains on EF-Tu.
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2.
  • Abdulkarim, Farhad, et al. (author)
  • Mutations to kirromycin resistance occur in the interface of domains I and III of EF-Tu.GTP
  • 1994
  • In: FEBS Letters. - : Wiley. - 0014-5793 .- 1873-3468. ; 352, s. 118-122
  • Journal article (peer-reviewed)abstract
    • The antibiotic kirromycin inhibits protein synthesis by binding to EF-Tu and preventing its release from the ribosome after GTP hydrolysis.We have isolated and sequenced a collection of kirromycin resistant tuf mutations and identified thirteen single amino acid substitutions at sevendifferent sites in EF-Tu. These have been mapped onto the 3D structures of EF-Tu’GTP and EF-Tu.GDP. In the active GTP form of EF-Tu themutations cluster on each side of the interface between domains I and III. We propose that this domain interface is the binding site for kirromycin.
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  • Ahlberg, Per E. (author)
  • Coelacanth fins and evolution
  • 1992
  • In: Nature. - 0028-0836 .- 1476-4687. ; 358, s. 459-
  • Journal article (peer-reviewed)
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Gerdin, Bengt, 1947- (127)
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Nordblad, P (80)
LUNDGREN, L (58)
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