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- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- 2008
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In: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
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Research review (peer-reviewed)abstract
- Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
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| 2. |
- Murari, A., et al.
(author)
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JET new diagnostic capability on the route to ITER
- 2007
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In: Fusion engineering and design. - : Elsevier BV. - 0920-3796 .- 1873-7196. ; 82:14-maj, s. 1161-1166
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Journal article (peer-reviewed)abstract
- The JET scientific programme is directed towards the development of ITER relevant scenarios. In support of this, significant effort has been made to develop diagnostics to better characterise the power deposition on the plasma facing components, to investigate in more detail the radiation losses particularly in the divertor region and to better detect Magneto Hydrodynamic Modes (MHD) instabilities and their effects on fast ion confinement. A new wide-angle infrared camera provides for the first time the opportunity to perform infrared thermography in the JET main chamber, even during fast events like ELMs and disruptions. A completely new bolometric system, with better spatial resolution particularly in the divertor, is now used to investigate the total radiation losses and their influence on the ELM behaviour. A new set of microwave waveguides has improved by 20 dB the signal to noise ratio of the JET X-mode reflectometers, that are now routinely used to detect MHD instabilities and in particular to localise the location of Alfven Eigenmodes. This improved diagnostic capability to monitor MHD instabilities is complemented by two new diagnostics to detect lost fast particles. Both the new scintillator probe and a poloidal array of Faraday cups have already shown clear correlations between MHD activity and ion losses at the edge.
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