| 1. |
- Sandén, Emma, et al.
(author)
-
Aberrant immunostaining pattern of the CD24 glycoprotein in clinical samples and experimental models of pediatric medulloblastomas
- 2015
-
In: Journal of Neuro-Oncology. - : Springer Science and Business Media LLC. - 0167-594X .- 1573-7373. ; 123:1, s. 1-13
-
Journal article (peer-reviewed)abstract
- The CD24 glycoprotein is a mediator of neuronal proliferation, differentiation and immune suppression in the normal CNS, and a proposed cancer biomarker in multiple peripheral tumor types. We performed a comparative analysis of CD24 gene expression in a large cohort of pediatric and adult brain tumors (n = 813), and further characterized protein expression in tissue sections (n = 39), primary brain tumor cultures (n = 12) and a novel orthotopic group 3 medulloblastoma xenograft model. Increased CD24 gene expression was demonstrated in ependymomas, medulloblastomas, anaplastic astrocytomas and glioblastomas, although medulloblastomas displayed higher expression than all other tumor entities. Preferential expression of CD24 in medulloblastomas was confirmed at protein level by immunostaining and computerized image analysis of cryosections. Morphologies and immunophenotyping of CD24(+) cells in tissue sections tentatively suggested disparate functions in different tumor subsets. Notably, protein staining of medulloblastoma cells was associated with prominent cytoplasmic and membranous granules, enabling rapid and robust identification of medulloblastoma cells in clinical tissue samples, as well as in experimental model systems. In conclusion, our results implicate CD24 as a clinically and experimentally useful medulloblastoma immunomarker. Although our results encourage further functional studies of CD24 as a potential molecular target in subsets of brain tumors, the promiscuous expression of CD24 in vivo highlights the importance of specificity in the future design of such targeted treatment.
|
|
| 2. |
- Andersson, Lena, 1965-, et al.
(author)
-
Respiratory health and inflammatory markers : Exposure to respirable dust and quartz and chemical binders in Swedish iron foundries
- 2019
-
In: PLOS ONE. - : PLOS. - 1932-6203. ; 14:11
-
Journal article (peer-reviewed)abstract
- PURPOSE: To study the relationship between respirable dust, quartz and chemical binders in Swedish iron foundries and respiratory symptoms, lung function (as forced expiratory volume FEV1 and vital capacity FVC), fraction of exhaled nitric oxide (FENO) and levels of club cell secretory protein 16 (CC16) and CRP.METHODS: Personal sampling of respirable dust and quartz was performed for 85 subjects in three Swedish iron foundries. Full shift sampling and examination were performed on the second or third day of a working week after a work free weekend, with additional sampling on the fourth or fifth day. Logistic, linear and mixed model analyses were performed including, gender, age, smoking, infections, sampling day, body mass index (BMI) and chemical binders as covariates.RESULTS: The adjusted average respirable quartz and dust concentrations were 0.038 and 0.66 mg/m3, respectively. Statistically significant increases in levels of CC16 were associated with exposure to chemical binders (p = 0.05; p = 0.01) in the regression analysis of quartz and respirable dust, respectively. Non-significant exposure-responses were identified for cumulative quartz and the symptoms asthma and breathlessness. For cumulative chemical years, non-significant exposure-response were observed for all but two symptoms. FENO also exhibited a non significant exposure-response for both quartz and respirable dust. No exposure-response was determined for FEV1 or FVC, CRP and respirable dust and quartz.CONCLUSIONS: Our findings suggest that early markers of pulmonary effect, such as increased levels of CC16 and FENO, are more strongly associated with chemical binder exposure than respirable quartz and dust in foundry environments.
|
|
| 3. |
- Burguillos Garcia, Miguel, et al.
(author)
-
Microglia-Secreted Galectin-3 Acts as a Toll-like Receptor 4 Ligand and Contributes to Microglial Activation.
- 2015
-
In: Cell Reports. - : Elsevier BV. - 2211-1247. ; 10:9, s. 1626-1638
-
Journal article (peer-reviewed)abstract
- Inflammatory response induced by microglia plays a critical role in the demise of neuronal populations in neuroinflammatory diseases. Although the role of toll-like receptor 4 (TLR4) in microglia's inflammatory response is fully acknowledged, little is known about endogenous ligands that trigger TLR4 activation. Here, we report that galectin-3 (Gal3) released by microglia acts as an endogenous paracrine TLR4 ligand. Gal3-TLR4 interaction was further confirmed in a murine neuroinflammatory model (intranigral lipopolysaccharide [LPS] injection) and in human stroke subjects. Depletion of Gal3 exerted neuroprotective and anti-inflammatory effects following global brain ischemia and in the neuroinflammatory LPS model. These results suggest that Gal3-dependent-TLR4 activation could contribute to sustained microglia activation, prolonging the inflammatory response in the brain.
|
|
| 4. |
- Freitag, C., et al.
(author)
-
Visualizing the entire DNA from a chromosome in a single frame
- 2015
-
In: Biomicrofluidics. - : AIP Publishing. - 1932-1058. ; 9:4
-
Journal article (peer-reviewed)abstract
- The contiguity and phase of sequence information are intrinsic to obtain complete understanding of the genome and its relationship to phenotype. We report the fabrication and application of a novel nanochannel design that folds megabase lengths of genomic DNA into a systematic back-and-forth meandering path. Such meandering nanochannels enabled us to visualize the complete 5.7 Mbp (1mm) stained DNA length of a Schizosaccharomyces pombe chromosome in a single frame of a CCD. We were able to hold the DNA in situ while implementing partial denaturation to obtain a barcode pattern that we could match to a reference map using the Poland-Scheraga model for DNA melting. The facility to compose such long linear lengths of genomic DNA in one field of view enabled us to directly visualize a repeat motif, count the repeat unit number, and chart its location in the genome by reference to unique barcode motifs found at measurable distances from the repeat. Meandering nanochannel dimensions can easily be tailored to human chromosome scales, which would enable the whole genome to be visualized in seconds. (C) 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
|
|
| 5. |
- Hudson, Lawrence N, et al.
(author)
-
The database of the PREDICTS (Projecting Responses of Ecological Diversity In Changing Terrestrial Systems) project
- 2017
-
In: Ecology and Evolution. - : John Wiley & Sons. - 2045-7758. ; 7:1, s. 145-188
-
Journal article (peer-reviewed)abstract
- The PREDICTS project-Projecting Responses of Ecological Diversity In Changing Terrestrial Systems (www.predicts.org.uk)-has collated from published studies a large, reasonably representative database of comparable samples of biodiversity from multiple sites that differ in the nature or intensity of human impacts relating to land use. We have used this evidence base to develop global and regional statistical models of how local biodiversity responds to these measures. We describe and make freely available this 2016 release of the database, containing more than 3.2 million records sampled at over 26,000 locations and representing over 47,000 species. We outline how the database can help in answering a range of questions in ecology and conservation biology. To our knowledge, this is the largest and most geographically and taxonomically representative database of spatial comparisons of biodiversity that has been collated to date; it will be useful to researchers and international efforts wishing to model and understand the global status of biodiversity.
|
|
| 6. |
- Høibakk, Ralph, et al.
(author)
-
On some power means and their geometric constructions
- 2018
-
In: Mathematica Æterna. - : Hilaris Ltd. - 1314-3336 .- 1314-3344. ; 8:3, s. 139-158
-
Journal article (peer-reviewed)abstract
- The main aim of this paper is to further develop the recently initiatedresearch concerning geometric construction of some power means wherethe variables are appearing as line segments. It will be demonstratedthat the arithmetic mean, the harmonic mean and the quadratic meancan be constructed for any number of variables and that all power meanswhere the number of variables are n = 2m, m 1 2 N for all powersk = 2?q and k = 2q; q 2 N can be geometrically constructed.
|
|
| 7. |
- Karlsson, Johanna, et al.
(author)
-
Att ta tillvara och bygga vidare på tidigare kunskaper (ämnesspecifik text: bild)
- 2018
-
Other publication (pop. science, debate, etc.)abstract
- För att kunna utmana elever på åldersadekvat nivå behöver lärare känna till elevernas tidigare kunskaper och erfarenheter. Resultatet från den inledande bedömningen (Kartläggningsmaterialet steg 1 och 2) ger en del information, men bedömningen behöver kompletteras med korta inledande kartläggningar inför varje arbetsmoment. För en bildlärare med nyanlända elever i elevgruppen handlar det om att bygga vidare på den ämneskunskap som eleven har med sig in i klassrummet och att med den som utgångspunkt kunna bedriva en språk- och kunskapsutvecklande undervisning som är kognitivt utmanande för alla elever.
|
|
| 8. |
- Karlsson, Johanna, et al.
(author)
-
Skrivutveckling i alla ämnen (ämnesspecifik text: bild)
- 2018
-
Other publication (pop. science, debate, etc.)abstract
- För att stötta nyanlända elever i skriftliga uppgifter i bildämnet kan läraren använda sig av cirkelmodellens fyra steg i textframställningen. Genom att gemensamt bygga upp ämneskunskaper, studera den texttyp som ska skrivas och formulera en första text ges eleven bättre möjligheter att klara en individuell skrivuppgift. Andra konkreta redskap som skrivmallar och begreppslistor fungerar också stöttande i skrivundervisningen
|
|
| 9. |
|
|
| 10. |
- Kaufmann, Tobias, et al.
(author)
-
Common brain disorders are associated with heritable patterns of apparent aging of the brain
- 2019
-
In: Nature Neuroscience. - : Nature Publishing Group. - 1097-6256 .- 1546-1726. ; 22:10, s. 1617-
-
Journal article (peer-reviewed)abstract
- Common risk factors for psychiatric and other brain disorders are likely to converge on biological pathways influencing the development and maintenance of brain structure and function across life. Using structural MRI data from 45,615 individuals aged 3-96 years, we demonstrate distinct patterns of apparent brain aging in several brain disorders and reveal genetic pleiotropy between apparent brain aging in healthy individuals and common brain disorders.
|
|
