| 1. |
- Pascher, R, et al.
(author)
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Computer-assisted 3D analysis of cell distributions in the normal and epileptic cerebral cortex: description of a methodology in progress.
- 1993
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In: Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society. - 0895-6111. ; 17:4-5, s. 405-10
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Journal article (peer-reviewed)abstract
- This paper describes software routines that (a) visualizes a stack of several thousands of aligned sequential photographic two-dimensional (2D) images stored in an image processing system; (b) creates a data base containing information about objects identified sequentially from the 2D images; (c) transfers the data base to a graphical terminal; (d) reconstructs a three-dimensional (3D) object space; and (e) supports on-line interaction between the image processing system and the graphical terminal. As an application example, the cell content of a prism of motor cerebral cortex of the cat is reconstructed. Preliminary results from reconstructing human epileptic temporal cortex (cortical microdysgenesia) are also reported.
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| 2. |
- Skoglund, Thomas, 1969, et al.
(author)
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3D reconstruction of biological objects from sequential image planes--applied on cerebral cortex from cat.
- 1993
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In: Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society. - 0895-6111. ; 17:3, s. 165-74
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Journal article (peer-reviewed)abstract
- A prism of cat cerebral cortex was reconstructed with a method for three-dimensional (3D) representation of biological objects. A series of 918 semithin sections were digitized into an image analysis system. The images were aligned and analyzed, and a data base with the coordinates and a classification of the cells was created. The data base (i.e., the cortical prism) was visualized in a 3D graphic terminal, and parameters such as columnar and lamellar organization, clustering, and cell density were analyzed. A neuronal perikaryon and its neurites was reconstructed and shown together with the cortical prism.
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| 3. |
- Skoglund, Thomas, 1969, et al.
(author)
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A method for 2D reconstruction of intracellularly labeled neurons from sequential sections.
- 1994
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In: Journal of neuroscience methods. - 0165-0270. ; 53:2, s. 199-202
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Journal article (peer-reviewed)abstract
- A technique for 2D reconstruction of intracellularly labeled neurons from sequential sections is described. The system consists of a Charged Coupled Device-camera mounted on a microscope, a videomixer and a IBM-compatible PC with a framegrabber. The neurons (interneurons from the spinal cord of the cat) were labeled iontophoretically by horshradish peroxidase and subsequently cut in 60 microns sections. The sections were aligned using the video mixer by fitting the cut dendrites and axon from one section with their counterparts in the following section. The images were then digitized in the PC where they were fused to create a superimposed picture of the aligned parts of the neuron; a 2D reconstruction was created.
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