| 1. |
- Pettkó-Szandtner, Aladár, et al.
(author)
-
Activation of an alfalfa cyclin-dependent kinase inhibitor by calmodulin-like domain protein kinase.
- 2006
-
In: Plant Journal. - 0960-7412. ; 46:1, s. 111-23
-
Journal article (peer-reviewed)abstract
- Kip-related proteins (KRPs) play a central role in the regulation of the cell cycle and differentiation through modulation of cyclin-dependent kinase (CDK) functions. We have identified a CDK inhibitor gene from Medicago truncatula (Mt) by a yeast two-hybrid screen. The KRPMt gene was expressed in all plant organs and cultured cells, and its transcripts accumulated after abscisic acid and NaCl treatment. The KRPMt protein exhibits seven conserved sequence domains and a PEST motif that is also detected in various Arabidopsis KRPs. In the yeast two-hybrid test, the KRPMt protein interacted with CDK (Medsa;CDKA;1) and D-type cyclins. However, in the pull-down assays, B-type CDK complexes were also detectable. Recombinant KRPMt differentially inhibited various alfalfa CDK complexes in phosphorylation assays. The immunoprecipitated Medsa;CDKA;1/A;2 complex was strongly inhibited, whereas the mitotic Medsa;CDKB2;1 complex was the most sensitive to inhibition. Function of Medsa;CDKB1;1 complex was not inhibited by the KRPMt protein. The mitotic Medsa;CYCB2 and Medsa;CYCA2;1 complexes responded weakly to this inhibitor protein. Kinase complexes from G2/M cells showed increased sensitivity towards the inhibitor compared with those isolated from G1/S-phase cells. In vitro phosphorylation of Medicago retinoblastoma-related protein was also reduced in the presence of KRPMt. Phosphorylation of this inhibitor protein by the recombinant calmodulin-like domain protein kinase (MsCPK3) resulted in enhanced inhibition of CDK function. The data presented emphasize the selective sensitivity of various cyclin-dependent kinase complexes to this inhibitor protein, and suggest a role for CDK inhibitors and CPKs in cross-talk between Ca2+ signalling and regulation of cell-cycle progression in plants.
|
|
| 2. |
- Sutton, Mark A., et al.
(author)
-
Dynamics of ammonia exchange with cut grassland : Synthesis of results and conclusions of the GRAMINAE Integrated Experiment
- 2009
-
In: Biogeosciences. - Goettingen : Nicolaus Copernicus University Press. - 1726-4170 .- 1726-4189. ; 6:12, s. 2907-2934
-
Journal article (peer-reviewed)abstract
- Improved data on biosphere-atmosphere exchange are fundamental to understanding the production and fate of ammonia (NH3) in the atmosphere. The GRAMINAE Integrated Experiment combined novel measurement and modelling approaches to provide the most comprehensive analysis of the interactions to date. Major intercomparisons of micrometeorological parameters and NH3 flux measurements using the aerodynamic gradient method and relaxed eddy accumulation (REA) were conducted. These showed close agreement, though the REA systems proved insufficiently precise to investigate vertical flux divergence. Grassland management had a large effect on fluxes: emissions increased after grass cutting (−50 to 700 ng m-2 s-1 NH3) and after N-fertilization (0 to 3800 ng m-2 s -1) compared with before the cut (−60 to 40 ng m-2 s -1). © Author(s) 2009.
|
|
| 3. |
|
|
| 4. |
- Szász, Robert-Zoltán, et al.
(author)
-
Computation of the acoustic field in an annular gas turbine combustion chamber using a hybrid approach
- 2006
-
In: Proceedings of the ASME Turbo Expo. - : ASME. ; 6 PART B, s. 1615-1624, s. 363-370, s. 1615-1624
-
Conference paper (peer-reviewed)abstract
- The acoustic field generated by flow unsteadiness in a model annular gas turbine (GT) combustion chamber is determined using a hybrid approach. In the flow solver the semi-compressible Navier-Stokes equations are resolved using LES as turbulence model. The acoustic solver is based on an inhomogeneous wave equation, where the instantaneous source terms are computed from the LES data in each time-instant. The flow and the acoustics in a GT combustor with co- and counter-rotating swirler burners have been considered. The results have shown that significant differences can be observed between the co- and counter-rotating configurations both in the flow and acoustic fields. Copyright
|
|
