| 1. |
- Adewumi, Oluseun, et al.
(author)
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Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
- 2007
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In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 25:7, s. 803-816
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Journal article (peer-reviewed)abstract
- The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
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| 2. |
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"Future Is Not What It Appears To Be" : pedagogy, genealogy and political epistemology : in honor and in memory of Kenneth Hultqvist
- 2006. - 1
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Editorial collection (other academic/artistic)abstract
- This book honors and is in memorial to Kenneth Hultqvist who helped initiate the broader research project to which this volume contributes. This book examines changes in the politics of knowledge in modern social life. The contributors provide important interventions in contemporary discussions about power, change, and the challenges that were raised in the Enlightenment about knowledge, reason, and the possibilities of humanity. The studies in the book examine a range of social programs and educational reforms to understand their implications, assumptions and consequences. Firstly, the chapters contribute to an international discussion on the historical relationships between social change, political rationalities, and the technologies of the self in a range of social life that included pedagogy. Secondly, the book offers new ways of studying and understanding the relation of the welfare state, social and education sciences, and todays configurations of liberal governing. Thirdly, the discussion brings into focus and critiques the distinctions of social and educational theories that differentiate "context" and programs, divides discourse from the "real", separates the subjective and objective and the personal and the public, and disconnects civil society from the state "reason" in modern government. "Trots sin ojämnhet är boken väl värd att läsa. Ansatsen bidrar till att skapa utrymme för reflektion kring vår samtid och de sätt varpå vi talar om skola, utbildning och framtid. Hur kommer det sig att vi talar om ett kunskapssamhälle? Vad har gjort ett sådant talesätt möjligt och vad för effekter i form av exkluderingspraktiker har det? Det är frågor som kan relateras till den förhoppning författarna har om att boken och det forskningsprojekt boken är kopplad till skall leda till 'an expanded, problematizing discussion of ongoing changes in the politics of knowledge in modern social life' (s 17). En god ambition som andas kritik i form av exempel i motsats till en mer grundläggande och föreskrivande kritik, något som inte är allt för vanligt i utbildningsvetenskaplig forskning idag."
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| 3. |
- Johansson, Åsa, et al.
(author)
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Common variants in the JAZF1 gene associated with height identified by linkage and genome-wide association analysis
- 2009
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In: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 18:2, s. 373-380
-
Journal article (peer-reviewed)abstract
- Genes for height has gained interest for decades, but only recently have candidate genes started to be identified. We have performed linkage analysis and genome-wide association for height in approximately 4,000 individuals from five European populations. A total of 5 chromosomal regions showed suggestive linkage and in one of these regions, two SNPs (rs849140 and rs1635852) were associated with height (nominal p=7.0 x 10(-8) and p=9.6 x 10(-7) respectively). In total, five SNPs across the genome showed an association with height that reached the threshold of genome-wide significance (nominal p<1.6 x 10(-7)). The association with height was replicated for two SNPs (rs1635852 and rs849140) using three independent studies (N=31,077, N=1,268 and N=5,746) with overall meta p-values of 9.4x10(-10) and 5.3x10(-8). These SNPs are located in the JAZF1 gene, which has recently been associated with type II diabetes, prostate and endometrial cancer. JAZF1 is a transcriptional repressor of NR2C2, which results in low IGF1 serum concentrations, perinatal and early postnatal hypoglycaemia and growth retardation when knocked-out in mice. Both the linkage and association analyses independently identified the JAZF1 region affecting human height. We have demonstrated, through replication in additional independent populations, the consistency of the effect of the JAZF1 SNPs on height. Since this gene also has a key function in the metabolism of growth, JAZF1 represents one of the strongest candidates influencing human height so far identified.
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| 4. |
- Rich, Rebecca L., et al.
(author)
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A global benchmark study using affinity-based biosensors
- 2009
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In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 386:2, s. 194-216
-
Journal article (peer-reviewed)abstract
- To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
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| 5. |
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The Future Is Not What It Appears To Be. : Pedagogy, Genealogy and Political Epistemology.
- 2006
-
Editorial collection (other academic/artistic)abstract
- This book examines changes in of knowledge in modern social life. The contributors provide important intinterventions in contemporary discussions about power, change, and the challenges that were raised in the Enlightenment about knowledge, reason, and the possibilities of humanity. The studies in the book examines programs and crucaial reforms to understand their implications, assumptions and concequences. Firstly the chapters contribute to an international discussion one the historical relationships between social change, political rationality and the technologies of the self in a range of social life that included pedagogy. Secondly, the book offers new ways of studying and understanding the relation of the welfare state, social and educational sciences, and todays configurations of liberal governing. Thirdly, the discussion brings into focus and critiques the distinctions of social and educational theories that differentiate "context" and programs, divides discourse from the ""real", separates the subjective and objecttive and the personal and the public, and disconnects civil society from the "reason" in modern government.
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