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- Hamberg, U, et al.
(author)
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Human kininogen from Cohns Fraction IV : comparisons of antigenicity and multiple forms
- 1979
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In: Advances in Experimental Medicine and Biology. - 0065-2598 .- 2214-8019. ; 120B, s. 173-183
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Journal article (peer-reviewed)abstract
- Kininogen was isolated from Cohns fraction IV by DEAE-chromatography, gel filtration and ammonium sulphate precipitation. Immunologically pure kininogen was prepared by removal of protein impurities using specific immunoadsorbents with Sepharose-bound antibody. Anti-kininogen serum was raised in rabbits against the pure antigen. Comparison with anti-kininogen sera prepared with the biologically active LMW antigen from whole plasma suggested antigenic identity by double immunodiffusion analysis. The Cohn-kininogen was shown to contain mainly two components (85%) in about equal amounts focusing with peaks at pI 4.2 (42%) and pI 4.3 (43%). These represent apparently structurally altered forms of the native plasma kininogen focusing at pI 4.5-4.6 (54%), which occurred as a minor component (13%).
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