1. |
- Abubaker, Jamal, et al.
(author)
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Bacterial community structure and microbial activity in different soils amended with biogas residues and cattle slurry
- 2013
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 72, s. 171-180
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Journal article (peer-reviewed)abstract
- Anaerobic digestion of organic materials generates residues of differing chemical composition compared to undigested animal manures, which may affect the soil microbial ecosystem differently when used as fertilizers. This study investigated the effects of two biogas residues (BR-A and BR-B) and cattle slurry (CS) applied at rates corresponding to 70 kg NH4+-N ha(-1) on bacterial community structure and microbial activity in three soils of different texture (a sandy, a clay and an organic clay soil). 16S rRNA genes were targeted in PCR reactions and bacterial community profiles visualized using terminal restriction fragment length polymorphism. General microbial activity was measured as basal respiration (B-resp), substrate-induced respiration (SIR), specific growth rate (mu(SIR)), metabolic quotient (qCO(2)) and nitrogen mineralization capacity (NMC). Non-metric multidimensional scaling analysis visualized shifts in bacterial community structure related to microbial functions. There were significant differences in bacterial community structure after 120 days of incubation (+20 degrees C at 70% of WHC) between non-amended (control) and amended soils, especially in the sandy soil, where CS caused a more pronounced shift than biogas residues. Terminal-restriction fragment (TRF) 307, the predominant peak in CS-amended sandy soil, was identified as possibly Bacillus or Streptococcus. TRF 226, the dominant peak in organic soil amended with BR-B, was classified as Rhodopseudomonas. B-resp significantly increased and SIR decreased in all amendments to organic soil compared with the control, potentially indicating decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass. This was also reflected in an elevated qCO(2) in the organic soil. The mu(SIR) level was higher in the sandy soil amended with BR-A than with BR-B or CS, indicating a shift toward species capable of rapidly utilizing glucose. NMC was significantly elevated in the clay and organic soils amended with BR-A and BR-B and in the sandy soil amended with BR-B and CS. Thus, biogas residues and cattle slurry had different effects on the bacterial community structure and microbial activity in the three soils. However, the effects of biogas residues on microbial activities were comparable in magnitude to those of cattle slurry and the bacterial community structure was less affected. Therefore, we do not see any reason not to recommend using biogas residues as fertilizers based on the results presented. (C) 2013 Elsevier B.V. All rights reserved.
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2. |
- Andersen, Christian B., et al.
(author)
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Pythium oligandrum induces growth promotion in starch potato without significantly altering the rhizosphere microbiome
- 2024
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In: Applied Soil Ecology. - 0929-1393 .- 1873-0272. ; 199
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Journal article (peer-reviewed)abstract
- Plant health promoting organisms, including microbial biological control agents, are of increasing importance for the development of more sustainable agriculture. To understand the function of these microbes as biological control agents under field conditions and their overall impact on soil and plant health, we need to learn more about the impact of plant beneficial microbes on the rhizosphere microbiome of crops such as potato. The plant beneficial oomycete Pythium oligandrum has previously been reported both as a biocontrol agent and as a plant growth promoter, or biostimulant, in several crop species. To investigate the potential of P. oligandrum as a biostimulant in potato, we performed a series of controlled-environment bioassays in three cultivars. We showed that biostimulation of potato by P. oligandrum is plant genotype-specific. We confirmed the biostimulation by P. oligandrum in the starch potato cultivar Kuras under field conditions. We further investigated the effects of P. oligandrum on the potato rhizosphere microbiome, sampling individual potato plants at three time points over the growing season (representing the vegetative growth phase, flowering, and the onset of senescence). Metabarcoding using ITS and 16S amplicon sequencing revealed no significant overall effect of P. oligandrum application on the bacterial and fungal rhizosphere communities. However, some genera were significantly differentially abundant after P. oligandrum application, including some classified as plant-beneficial microbes. We conclude that P. oligandrum has a cultivar-dependent growth-promoting effect in potato and only minor effects on the rhizosphere microbiome.
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3. |
- Arrazola Vasquez, Elsa, et al.
(author)
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Earthworm burrowing modes and rates depend on earthworm species and soil mechanical resistance
- 2022
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 178
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Journal article (peer-reviewed)abstract
- Earthworms drive multiple soil processes, but their specific impact on soil functions differs between earthworm species and ecological categories. A key challenge in modern agriculture is soil compaction due to heavy ma-chinery, but we have limited quantitative knowledge about how the burrowing activity of different earthworm species is affected by compaction. Here, we address this question in a laboratory experiment with 2-D terraria, where we used Aporrectodea caliginosa (Savigny, 1826) and Aporrectodea longa (Ude, 1885) as representatives of two different ecological categories. We exposed both species to four different soil mechanical resistance levels and monitored their burrowing activity for three days. We quantified burrowing rates and cast production, assessed the burrowing mode, and estimated energy requirements as a function of soil mechanical resistance. The results showed that the burrowing rates of both earthworm species significantly decreased with increasing soil mechanical resistance, but that the impact was species-dependent and lower for A. longa. Earthworms changed their burrowing mode towards ingestion when soil mechanical resistance increased, and this shift was more prominent for A. caliginosa that primarily burrowed via cavity expansion (i.e. by pushing soil aside) at low soil mechanical resistance. We further show that energy requirement and cast produced per unit burrow length increased with soil mechanical resistance. Our study revealed significant and species-dependent adverse effects of soil mechanical resistance on earthworm burrowing, which in turn has consequences for many soil processes mediated by earthworms, such as water infiltration, soil aeration, nutrient cycling and soil organic matter turnover.
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4. |
- Ayuke, Fredrick, et al.
(author)
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Effects of biocontrol bacteria and earthworms on the severity of Alternaria brassicae disease and the growth of oilseed rape plants (Brassica napus)
- 2017
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 117-118, s. 63-69
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Journal article (peer-reviewed)abstract
- Biological control of plant diseases through the addition of microbial biocontrol agents and the promotion of earthworms can be an environmentally friendly alternative to the chemical control of plant diseases. However, possible risks with biocontrol agents and their interactions with earthworms and other soil biota have not been well studied. The aim of this study was to assess whether the beneficial bacterium Bacillus amyloliquefaciens and the earthworms Aporrectodea caliginosa or Aporrectodea longa could reduce disease in oilseed rape (Brassica napus) challenged with the pathogen Alternaria brassicae. Plant growth and productivity were measured as plant survival, height, biomass, and flower development as well as disease index. A second objective was to assess whether the presence of the bacterium at high concentrations would influence the survival, growth, and reproduction of the earthworms. One outdoor and one greenhouse experiment were performed with Br. napus plants challenged with AL brassicae inoculated to the plant leaves in the presence or absence of Bacillus amyloliquefaciens inoculated to the root environment and in the presence or absence of earthworms (Ap. caliginosa or Ap. longa) added to the soil. All treatments were replicated three times. In the outdoor experiment, inoculation with AL brassicae reduced the growth of plants and the addition of Ap. caliginosa increased plant height. In the greenhouse experiment, pairwise comparisons of plants challenged with AL brassicae showed that treatment with B. amyloliquefaciens led to significantly lower disease index than the treatment with Ap. caliginosa plus B. amyloliquefaciens, while other treatments had intermediate disease indices. The addition of AL brassicae or B. amyloliquefaciens increased the survival and mass increment of Ap. caliginosa as a main effect when used separately but not when used in combination.This study did not give any clear indication of the usefulness of B. amyloliquefaciens for biocontrol of plant pathogens such as AL brassicae when growing plants in natural soil. In addition, no significantly positive effects from the tested earthworm species were seen.
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5. |
- Bargaz, Adnane, et al.
(author)
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Species interactions enhance root allocation, microbial diversity and P acquisition in intercropped wheat and soybean under P deficiency
- 2017
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 120, s. 179-188
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Journal article (peer-reviewed)abstract
- Belowground interactions in grain legume-cereal intercrops may improve resource acquisition and adaptation to environmental constraints such as phosphorus (P) deficiency. To advance the knowledge of belowground facilitative mechanisms involved in P-deficiency tolerance (root allocation, biochemical and microbial responses), soybean (Glycine max) and wheat (Triticum aestivum) were grown as monocrops and intercrops under P-deficiency and P-sufficiency conditions in soil-filled rhizoboxes. The hypothesis was that intercropping stimulates root microbial diversity, root biomass allocation and P-hydrolyzing acid phosphatases (APase) activity in roots under P-deficient conditions. Total root dry weight (RDW), length, and surface area significantly increased in P-deficient intercropped wheat and soybean. Greater root allocation to deeper soil layers was evident for P-deficient intercropped wheat. Shallow roots of intercropped wheat exhibited highly stimulated APase activity under P-deficient conditions while shallow roots of monocropped soybean exhibited higher APase activity in comparison to deeper roots, irrespective of P treatment. Root fungal diversity was significantly (p < 0.05) higher in intercropped wheat, and was significantly correlated with RDW, root APase activity, shoot P, and soil available P (rho = 0.24, p= 0.01). Root bacterial diversity was higher in both intercrops, and was significantly correlated with RDW and shoot N concentration. The observed shifts in root microbial diversity, root biomass allocation and APase activity provide explanatory mechanisms of relationships between rhizosphere heterogeneity and pathways for increased P acquisition in diversified crops. Advanced belowground metabolomics on root microbial communities are required to reveal the beneficial effect of root microorganisms in associations of different crop species.
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6. |
- Barreiro, Ana, et al.
(author)
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Soil bacteria respond to regional edapho-climatic conditions while soil fungi respond to management intensity in grasslands along a European transect
- 2022
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 170
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Journal article (peer-reviewed)abstract
- Soil microbial community structure is determined by environmental conditions and influenced by other factors, such as the intensity of the land use management. Studies addressing the effect of environmental factors and management on grassland soil microbial communities at the continental scale are missing, and the wide range of ecosystem services provided by these ecosystems are thus also wanting. To address this knowledge gap, this study presents data on grassland soil microbial communities along a pan-European agro-ecological gradient. The transect included five geographical locations (Sweden, Germany, Switzerland, Portugal mainland, Portugal Azores). At each location, soils were collected in two regions characterized by favourable and less favourable conditions for plant growth. In each of these ten regions, grasslands along a gradient of management intensity were selected, i.e. grassland under intensive, less intensive and extensive management. Phospholipid fatty acid analysis (PLFA) was used to characterize the microbial community structure (PLFA pattern) in relation to climatic and soil properties. Over the whole geographical range, the environmental properties determined the soil microbial community structure. In Sweden and Switzerland, the regional growth conditions had the strongest influence on the soil microbial communities, while in Germany, Portugal mainland and Azores the management intensity was more important. Splitting up this whole community response into individual groups reveals that, in general, saprotrophic fungal biomarkers were highest in extensively managed grasslands while bacterial biomarkers differed mainly between the regions. We conclude that at the transect level, climate and soil properties were the most important factors influencing soil bacterial community structure, while soil fungal groups were more responsive to grassland management intensity. Overall agricultural sustainability could benefit from informed soil health promoting management practices, and this study contributes to such knowledge, showing the importance of management for the soil microbial biomass and community structure.
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7. |
- Bengtsson, Jan
(author)
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The response of springtails to fire in the fynbos of the Western Cape, South Africa
- 2016
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 108, s. 165-175
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Journal article (peer-reviewed)abstract
- Although fire is a natural form of disturbance in many ecosystems, the frequency of fires is increasing due to human activities. Hence, understanding the impacts of fire on biodiversity and ecosystem functioning has become increasingly important. In this study we investigated the effects of a large-scale fire on an important soil-dwelling group, springtails (Collembola), one year before and for three consecutive years after a fire in the fire-prone fynbos ecosystem in South Africa. In particular, we investigate the resistance of the springtail assemblages (i.e. their ability to remain relatively unchanged in the face of a disturbance), and their resilience (i.e. ability to return to a pre-disturbance state). To do this we sampled two sites with contrasting vegetation (Erica and Protea) and used three different standardized litter types in litterbag traps. A total of 35 springtail species from 31 genera and 14 families was found. The springtail assemblages in this fynbos system showed slightly more resistance to fire than resilience after the fire event, though substantial variation was found among vegetation types. Mean species richness and abundance per litterbag varied among the Protea and Erica sites, with resistant species being dominant in the Erica site, while species that showed an increase after the fire were dominant in the Protea site. Differences were also found between life forms: atmobiotic (free-living in vegetation) and epiedaphic (surface dwelling) species showed a significant decline in mean species richness directly after the fire in the Erica site. Euedaphic (soil-dwelling) species richness remained unchanged post-fire in the Erica site, while actually slightly increasing after the fire in the Protea site. Although the fynbos springtail assemblages had not fully recovered to pre-fire abundance after three years, many species appear to be resistant to or recover rapidly after fires, at least as ascertained over the relatively short (four years) duration of the study. It is likely that this response is influenced by the presence of suitable refugia within sites and by species-specific traits. Given changing fire regimes and the increasing frequency of fires due to human disturbances, the system will likely become more dominated by resistant springtail species preferring nutrient rich circumstances and easily decomposed litters. (C) 2016 Elsevier B.V. All rights reserved.
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8. |
- Bisang, Irene, et al.
(author)
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Three decades of field surveys reveal a decline of arable bryophytes in the Swiss lowlands despite agri-environment schemes
- 2021
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In: Agriculture, Ecosystems & Environment. Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 313
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Journal article (peer-reviewed)abstract
- Agricultural intensification represents one of the major drivers for the dramatic loss of biodiversity worldwide. To halt the decline of farmland biodiversity, Switzerland adopted agri-environment schemes (AES) in 1998. Here, we monitored the occurrence, abundance and habitats of two species of arable bryophyte specialists, the Field hornwort (Anthoceros agrestis) and the Carolina hornwort (Phaeoceros carolinianus), in 28 crop fields in the intensively cultivated Swiss Plateau from 1991–2018, to investigate the effects of arable management, AES directives, and weather conditions on their performance. The target species are characteristic of the specialized short-lived arable bryophyte flora of Central Europe that depend on bare substrate in low-intensively cultivated and regularly ploughed fields. Trends in their occurrence thus reflect in many respects the status of the arable bryophytes in cultivated fields. Hornwort occurrence significantly declined between 1991 and 2018. A strong decrease in stubble fields that remain unmanaged after harvest, the favourite habitat for many arable specialists in the study area, largely accounted for the decline. Stubble fields nearly disappeared in the study area because of a gradual reduction in the cultivation area of cereals and the increasing practice of immediate post-harvest tillage. The latter is common in intensive arable farming and was accentuated by AES directives amended in 2005. Hornwort occurrences were positively affected by high air humidity during summer, but weather effects were subordinate to management effects. We propose tailored amendments of AES regulations, that aremented at selected sites, to maintain the characteristic arable specialist bryophytes in the Swiss Plateau: crop rotation with adequate proportions of cereals that are regularly ploughed but not before the end of October, no post-harvest processing of stubble fields, and optimization of the existing instrument ‘Biodiversity Promoting Areas’, e.g., short-term fallows in crop fields. Late-autumn or overwintering stubbles and short-term fallows will benefit many other organisms that depend on extensively managed open habitats, for example arableflowers, farmland breeding birds and specialized arthropods
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9. |
- Björsell, Pia, et al.
(author)
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Interactions between some plant-parasitic nematodes and Rhizoctonia solani in potato fields
- 2017
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 113, s. 151-154
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Journal article (peer-reviewed)abstract
- The pathogenic fungus Rhizoctonia solani causes major economic losses for potato producers in Sweden. Producers, as well as extension officers, have reported possible increases in severity of R. solani when free-living plant-parasitic nematodes are present and active. The aim of this study was to investigate possible interactions and spatial correlations between root-lesion nematodes (Pratylenchus spp.), stubby-root nematodes (Trichodoridae) and the severity of stem canker caused by the fungus R. solani under field conditions. Nematodes in the genus' Globodera were also included after finding high numbers of these potato cyst nematode juveniles in the samples. Soil samples were taken in eight potato fields, located in the middle part of Sweden, with observed outbreaks of soil-borne stem canker caused by R. solani. Grading of stem canker and soil collections for nematode extraction were performed along transects starting from the centre of field patches with observed stem canker. There was no difference in the number of nematodes within the patches for any of the investigated nematode taxa, but the severity of stem canker was higher on plants graded in the centre of the patches compared to those graded in the margins. In addition, there was a spatial correlation between R. solani and stubby-root nematodes as well as potato cyst nematodes, but not for root-lesion nematodes. These results show that there is an interaction between plant-parasitic nematodes and R. solani in the field and this knowledge is of importance for future decisions of appropriate management methods. (C) 2017 The Authors. Published by Elsevier B.V.
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10. |
- Campbell, Colin
(author)
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The arbuscular mycorrhizal fungus Glomus hoi can capture and transfer nitrogen from organic patches to its associated host plant at low temperature
- 2011
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In: Applied Soil Ecology. - : Elsevier BV. - 0929-1393 .- 1873-0272. ; 48, s. 102-105
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Journal article (peer-reviewed)abstract
- Arbuscular mycorrhizal fungi have been suggested to be of potential benefit in achieving sustainable agriculture systems. However, there is conflicting information on the degree to which arbuscular mycorrhizal fungi (AMF) can grow and function at soil temperatures typical of temperate regions. To resolve this conflict we grew Plantago lanceolata L inoculated with Glomus hoi (UY 110) in microcosm units maintained at 12/10 degrees C (day/night). The microcosms had two compartments, one planted and one not. The root-free compartment contained either an organic ((15)N:(13)C labelled milled shoot material) or a sand patch. When permitted access, G. hoi proliferated hyphae extensively in the organic patch material. Plant (15)N content was a simple function of length density of extra-radical mycelium (ERM) in the patch and c. 6% of host plant N was derived from the patch. These results indicate that G. hoi not only grew at these realistic soil temperatures, but also conferred a nutritional benefit to its host. (C) 2011 Elsevier B.V. All rights reserved.
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