| 1. |
- Duan, Maoli, et al.
(author)
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Imaging of the guinea pig cochlea following round window gadolinium application
- 2004
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In: NeuroReport. - : Wolters Kluwer. - 0959-4965 .- 1473-558X. ; 15:12, s. 1927-1930
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Journal article (peer-reviewed)abstract
- Precise, non-invasive determination of the aetiology and site of pathology of inner ear disorders is difficult. The aim of this study was to describe an alternative method for inner ear visualization, based on local application of the paramagnetic contrast agent gadolinium. Using a 4.7 T MRI scanner, high contrast images of all four cochlear turns were obtained 3.5 h after placing gadolinium on the round window membrane. Gadolinium cleared from the cochlea within 96 h. Auditory brainstem response measurements performed on a separate group of animals showed no significant threshold shifts after the application, indicating that gadolinium is non-toxic to the guinea pig cochlea.
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| 2. |
- Englund, Ulrica, et al.
(author)
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The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS
- 2000
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In: NeuroReport. - : Lippincott Williams & Wilkins. - 0959-4965 .- 1473-558X. ; 11:18, s. 3973-3977
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Journal article (peer-reviewed)abstract
- A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS.
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| 3. |
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| 4. |
- Holmberg, Johan, et al.
(author)
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Phobic postural vertigo: body sway during vibratory proprioceptive stimulation
- 2003
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In: NeuroReport. - : Ovid Technologies (Wolters Kluwer Health). - 1473-558X .- 0959-4965. ; 14:7, s. 1007-1011
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Journal article (peer-reviewed)abstract
- Phobic postural vertigo patients might rely more on proprioceptive than visual cues to regulate stance. We tested 14 phobic postural vertigo patients and 24 healthy subjects with posturography during quiet stance and periods of vibratory proprioceptive calf muscle stimulation, both with eyes open and closed. During quiet stance phobic postural vertigo patients showed higher torque variance than healthy subjects, especially above 0.1 Hz. Vibratory proprioceptive stimulation increased the differences between healthy subjects and phobic postural vertigo patients. The patients were less able to use vision to counteract vibration-induced movements. Phobic postural vertigo patients are more sensitive to proprioceptive disturbances than healthy subjects are, and less apt to use visual information to control upright stance. This might be part of an anxious mode of balance control.
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| 5. |
- Jiang, Chonghe, 1951-, et al.
(author)
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Cooling of the urinary bladder activates neurons in the dorsal horn of the spinal cord
- 2004
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In: NeuroReport. - : Ovid Technologies (Wolters Kluwer Health). - 0959-4965 .- 1473-558X. ; 15:2, s. 351-355
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Journal article (peer-reviewed)abstract
- Although visceral innocuous cold receptors have been documented, the central termination of their afferents is unknown. We used menthol solution (0. 6 m M) to obtain selective activation of cold receptors in the urinary bladder of rats. Innocuous cold stimulation induced Fos expression in a population of neurons in the superficial dorsal horn of L6-SI segments of the spinal cord. Neurons in other regions of the spinal cord, e.g. the lumbar parasympathetic nucleus or the dorsal commissure region, were activated to a similar degree by menthol and control infusions, indicating a response to bladder filling. Our results are consistent with the proposal that subsets of modality-specific dorsal horn neurons convey specific information regarding the exteroceptive and interoceptive state of the animal. © 2004 Lippincott Williams & Wilkins.
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| 6. |
- Paues, Jakob, et al.
(author)
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Feeding-related immune responsive brain stem neurons : association with CGRP
- 2001
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In: Neuroreport. - 0959-4965 .- 1473-558X. ; 12:11, s. 2399-2403
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Journal article (peer-reviewed)abstract
- Using dual-labeling in situ hybridization histochemistry, the neurotransmitter expression of immune-responsive neurons in the pontine parabrachial nucleus, a major relay for interoceptive information, was investigated. Intravenous injection of bacterial wall lipopolysaccharide resulted in dense c-fos mRNA expression in the external lateral parabrachial nucleus, and a majority of the c-fos expressing cells also expressed calcitonin gene-related peptide (CGRP) mRNA. In contrast CGRP-posi- tive cells in the adjoining external medial subnucleus were c-fos negative. Taken together with previous hodological and behavioral studies, these data suggest that CGRPergic parabrachial neurons may mediate lipopolysaccharide-induced anorexia by means of their projection to central nucleus of the amygdala.
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| 7. |
- Skogh, C, et al.
(author)
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Homotopic glial regulation of striatal projection neuron differentiation
- 2003
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In: NeuroReport. - : Ovid Technologies (Wolters Kluwer Health). - 1473-558X .- 0959-4965. ; 14:7, s. 1037-1040
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Journal article (peer-reviewed)abstract
- We have studied the differentiation of striatal projection neurons in co-culture with expanded glia from different regions of the embryonic mouse telencephalon. Our results show that when striatal progenitors are cultured on glia derived from the same region as they originate (i.e. the lateral ganglionic eminence), the neurons formed exhibit long processes. This is not the case when the cells are co-cultured with glia derived from the adjacent telencephalic region, the medial ganglionic eminence. Moreover, expression of the striatal projection neuron marker, dopamine- and cAMP-regulated phosphoprotein (DARPP-32) was significantly enhanced in neurons cultured on the homotopic glia. Thus, glial cultures derived from the lateral ganglionic eminence positively regulate the differentiation of striatal projection neurons in vitro.
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| 8. |
- Valenciano, Ana, et al.
(author)
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Biotin decreases retinal apoptosis and induces eye malformations in the early chick embryo
- 2002
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In: NeuroReport. - 0959-4965 .- 1473-558X. ; 13:3, s. 297-299
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Journal article (peer-reviewed)abstract
- Proliferation, cell death and differentiation occur simultaneously in developing retina and are precisely orchestrated. We have studied the effects of biotin (vitamin H) on early retinal development. In vivo administration of biotin to early embryonic chick eyes at moderately elevated levels induced malformations, affecting retina and lens structures. The effects were strictly age dependent and were only found in embryos treated between Hamburger and Hamilton stage 14-17. Biocytin, a biotin analogue, mimicked biotin effects, while avidin could block the effects. At the cellular level, biotin did not affect proliferation but reduced apoptosis. These results suggest that an adequate content of biotin and a precise regulation of retinal cell death are required for the correct morphogenesis of the eye.
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| 9. |
- Widerberg, Annika, et al.
(author)
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Tourniquet compression: a non-invasive method to enhance nerve regeneration in nerve grafts.
- 2002
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In: NeuroReport. - : Ovid Technologies (Wolters Kluwer Health). - 1473-558X .- 0959-4965. ; 13:4, s. 371-375
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Journal article (peer-reviewed)abstract
- One hindlimb of a rat was subjected to tourniquet compression (150, 200 and 300 mmHg; 2 h). After 6 days a 10 mm sciatic or tibial nerve graft from the compressed limb was sutured to bridge a 3-4 mm gap in the sciatic nerve of the non-compressed limb. The distances of regenerating sensory axons were measured 6 days post surgery (tibial grafts, 8 days). Compression at 200 and 300 mmHg led to significantly longer regeneration distances than those seen in controls. Incorporation of BrdU and expression of p75 receptor by non-neuronal cells (Schwann cells) in sciatic nerves 6 days after compression (150 and 300 mmHg; 2 h) was also increased as a sign of Schwann cell activation. Tourniquet compression may be used as a non-invasive method to enhance nerve regeneration in nerve grafts.
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| 10. |
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