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- Zhao, Y., et al.
(author)
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Bismuth nanoplatelets : universal synthetic strategy and emerging application for PEC-type photodetectors
- 2023
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In: MATERIALS TODAY NANO. - : Elsevier BV. - 2588-8420. ; 23
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Journal article (peer-reviewed)abstract
- Bi nanoplatelets (NPLs) with a narrow size distribution and high yield have been successfully fabricated by a novel two-step sonication-assisted solvothermal method. Applied as photo-electrochemical (PEC)-type photodetectors (PDs), it is shown that the Bi NPLs exhibit a pronounced photo-response ranging from the ultraviolet to the visible regions. The photo-response performance of Bi NPLs-based PDs was adjusted by external conditions and interpreted by density functional theory calculations in terms of the formation of the respective Bi-based compounds. The PEC results demonstrate that the optimal photocurrent density (P-ph) and photoresponsivity (R-ph) can reach 12.27 mu A/cm(2) and 3016 mu A/W in alkaline electrolytes, respectively. Besides, the Bi NPLs-based PDs exhibit superb long-term cycling stability (similar to 97.86% remained after 1000 cycles). We conclude that Bi NPLs demonstrate great promise as an emerging building block for the design of high-performance PEC-type PDs as well as other novel optoelectronic devices, holding the potential for breakthrough developments in these fields.
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- Fast, L, et al.
(author)
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Fluorescence-Based Measurements of Membrane-Bound Angiotensin Converting Enzyme 2 Activity Using Xenopus Laevis Oocytes
- 2022
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In: Biosensors. - : MDPI AG. - 2079-6374. ; 12:8
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Journal article (peer-reviewed)abstract
- Functional investigations of enzymes involving cellular expression systems are important for pharmacological studies. The precise control of expression is challenging in transiently transfected mammalian cell lines. Here, we explored the ability of Xenopus laevis oocytes to express a membrane-bound enzyme for functional characterization using standard 96-well plates and a fluorescence-based plate reader assay. We microinjected oocytes with cRNA encoding the angiotensin converting enzyme 2 (ACE2) and measured the enzymatic activity in single oocytes using a commercial fluorescence-based assay. The injected oocytes showed up to a 50-fold increase in fluorescence compared to uninjected oocytes. This fluorescence intensity was dose-dependent on the amount of ACE2 cRNA. These results suggest that Xenopus oocytes can be used for the functional evaluation of membrane-bound enzymes, decreasing the experimental workload.
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- Sobek, Jens, et al.
(author)
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Efficient characterization of multiple binding sites of small molecule imaging ligands on amyloid-beta, tau and alpha-synuclein
- 2024
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In: European Journal of Nuclear Medicine and Molecular Imaging. - : SPRINGER. - 1619-7070 .- 1619-7089.
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Journal article (peer-reviewed)abstract
- PurposeThere is an unmet need for compounds to detect fibrillar forms of alpha-synuclein (alpha Syn) and 4-repeat tau, which are critical in many neurodegenerative diseases. Here, we aim to develop an efficient surface plasmon resonance (SPR)-based assay to facilitate the characterization of small molecules that can bind these fibrils.MethodsSPR measurements were conducted to characterize the binding properties of fluorescent ligands/compounds toward recombinant amyloid-beta (A beta)42, K18-tau, full-length 2N4R-tau and alpha Syn fibrils. In silico modeling was performed to examine the binding pockets of ligands on alpha Syn fibrils. Immunofluorescence staining of postmortem brain tissue slices from Parkinson's disease patients and mouse models was performed with fluorescence ligands and specific antibodies.ResultsWe optimized the protocol for the immobilization of A beta 42, K18-tau, full-length 2N4R-tau and alpha Syn fibrils in a controlled aggregation state on SPR-sensor chips and for assessing their binding to ligands. The SPR results from the analysis of binding kinetics suggested the presence of at least two binding sites for all fibrils, including luminescent conjugated oligothiophenes, benzothiazole derivatives, nonfluorescent methylene blue and lansoprazole. In silico modeling studies for alpha Syn (6H6B) revealed four binding sites with a preference for one site on the surface. Immunofluorescence staining validated the detection of pS129-alpha Syn positivity in the brains of Parkinson's disease patients and alpha Syn preformed-fibril injected mice, 6E10-positive A beta in arcA beta mice, and AT-8/AT-100-positivity in pR5 mice.ConclusionSPR measurements of small molecules binding to A beta 42, K18/full-length 2N4R-tau and alpha Syn fibrils suggested the existence of multiple binding sites. This approach may provide efficient characterization of compounds for neurodegenerative disease-relevant proteinopathies.
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