| 1. |
|
|
| 2. |
- Andersson, Britta, 1979-
(author)
-
Manipulation of potassium ion fluxes to induce apoptosis in lung cancer cells
- 2007
-
Doctoral thesis (other academic/artistic)abstract
- Apoptosis is a special form of cell death that if non-functional may lead to diseases such as cancer. A reduction of the intracellular potassium ion (K+) content is necessary for activating enzymes important for the execution of apoptosis. Pharmacological modulation of K+ fluxes to reduce intracellular K+ in cancer cells might therefore force the cells into apoptosis and decrease tumour cell mass. Human malignant pleural mesothelioma (MPM) is a form of cancer often caused by asbestos exposure. Although asbestos has been banned in the Western World, the incidence of MPM is expected to increase. Cisplatin is the first-line chemotherapy for MPM, but acquired resistance to the drug is a clinical problem. This thesis is mainly based on work with the human malignant pleural mesothelioma cell line (P31 wt) and a cisplatin-resistant sub-line (P31 res). The aim was to first characterize K+ fluxes in P31 wt and P31 res cells, and then manipulate them in order to reduce intracellular K+ and induce apoptosis with K+ manipulation alone or in combination with cisplatin. Characterization of K+ fluxes in P31 wt cells showed that: 1) ouabain, a digitalis-like drug, and specific blocker of the Na+, K+, ATPase pump, effectively inhibited K+ uptake, 2) bumetanide, a diuretic, and an inhibitor of the Na+, K+, 2Cl-¬-cotransporter, had a transient effect on K+ uptake, and 3) the antifungal drug amphotericin B stimulated K+ efflux. In order to determine intracellular K+ content, the potassium-binding fluorescent probe PBFI-AM was used in a 96-well plate assay. After a 3-h incubation with ouabain, with or without bumetanide, combined with amphotericin B, the intracellular K+ content was reduced in P31 wt cells but not in P31 res cells. Ouabain induced apoptosis in both P31 wt and P31 res cells. P31 res cells were sensitized to cisplatin by ouabain, since 10 mg/L cisplatin in combination with ouabain induced about the same percentage of apoptotic cells as 40 mg/L cisplatin. Apoptosis was executed via caspase-3 activation in both P31 wt and P31 res cells. Amphotericin B enhanced ouabain-induced apoptosis in P31 wt cells via caspase-9 activation, with increased caspase-3 activation and DNA fragmentation as consequences. Ouabain-induced apoptosis in P31 res cells was executed via increased expression of pro-apoptotic Bak. The combination of cisplatin with ouabain and amphotericin B was stressful to both P31 wt and P31 res cells, since SAPK/JNK a known factor in stress-induced apoptosis was activated. In conclusion, K+ flux manipulation with clinical used drugs can induce apoptosis per se and also enhance cisplatin-induced apoptosis in P31 wt and P31 res cells.
|
|
| 3. |
|
|
| 4. |
- Andersson, Christian, et al.
(author)
-
Mobila strategier. En rapport från arbetsgruppen Nya medier
- 2007
-
Reports (other academic/artistic)abstract
- Utredningens fokus skall ligga på Bibliotek och ITs framtida hantering av mobila tjänster, läsplattor, ljud och bildfiler i olika format och dess tillgängliggöranden, MP3-spelare, dator- och TV-spel. Texten kan ses som en omvärldsanalys av möjligheter och scenarion kring arbetet med ovan nämnda tjänster och produkter. Förhoppningen är att texten ska peka på möjliga fokus för organisationen, stimulera till idéproduktion och vara avstampen för nya arbetstrupper för Bibliotek och IT.
|
|
| 5. |
- Andersson, Fia, 1950-
(author)
-
Att utmana erfarenheter : Kunskapsutveckling i en forskningscirkel
- 2007
-
Doctoral thesis (other academic/artistic)abstract
- This thesis aims at describing and analysing the process and content in knowledge development within a research circle. The participants in this circle are seven teachers who work with multilingual children diagnosed within the autism spectrum, and me as a researcher.The study is conducted within the tradition of participatory-oriented research. The research issue concerns the questions these teachers ask themselves in their everyday work. The study, consisting of twelve meetings, was carried out during 2004-2005. In the final meeting material collected in the circle was analysed together by all participants. The knowledge-content analysis disclosed four main themes: mother-tongue issues, disabilities and diagnoses, the issue of frames and matters related to working with parents and other professionals. The results show that once a child is diagnosed within the autism spectrum the diagnosis “takes over” and mother-tongue instruction is seldom discussed.The participants in the circle found it difficult to collaborate with the various authorities involved in working with a child and its family. They also found it difficult to communicate with parents, due to language barriers, different cultural contexts, and the observation that interpreters did not translate properly. The participants noted an existing hierarchy in relation to doctors and psychologists, regarded as having the mandate to assess a child’s ability and suggest placement in class.A conclusion is that the work of the teachers entails a high degree of complexity, and that knowledge meetings and collaboration between parents, teachers, and the various authorities are needed. During the circle process emancipating collective knowledge was constructed transcending what any participant had from the start. Experiences discussed in continuing dialogues, and in an on-going process, seem to be essential for generation of knowledge. When experiences were challenged, potentials for different actions were revealed.
|
|
| 6. |
|
|
| 7. |
- Håglin, Lena, et al.
(author)
-
Kost och hälsa i Sverige och världen
- 2006
-
In: Näringslära för högskolan. - : Liber AB, Stockholm. - 9147053550 ; , s. 29-57
-
Book chapter (pop. science, debate, etc.)
|
|
| 8. |
- Janson, Veronica, et al.
(author)
-
Acquisition of Cisplatin-resistance in Malignant Mesothelioma Cells Abrogates Na,K(+),2Cl(-;)-cotransport Activity and Cisplatin-induced Early Membrane Blebbing
- 2008
-
In: Cellular Physiology and Biochemistry. - : S. Karger. - 1015-8987 .- 1421-9778. ; 22:1-4, s. 45-56
-
Journal article (peer-reviewed)abstract
- AIMS: Resistance mechanisms are important limiting factors in the treatment of solid malignancies with cis-diamminedichloroplatinum(II) (cisplatin). To gain further understanding of the effects of acquired cisplatin-resistance, we compared a human malignant pleural mesothelioma cell line (p31) to a sub-line (p31res1.2) with acquired cisplatin-resistance.METHODS AND RESULTS: The role of Na(+),K(+),2Cl(-)-cotransport (NKCC1) activity in cisplatin-induced morphological changes and acquired cisplatin-resistance was investigated in a time-resolved manner. Acquisition of cisplatin-resistance resulted in markedly reduced NKCC1 activity, absence of cisplatin-induced early membrane blebbing, and increased basal caspase-3 activity. At equitoxic cisplatin concentrations, P31res1.2 cells had a faster activation of caspase-3 than P31 cells, but the end-stage cytotoxicity and number of cells with DNA fragmentation was similar. Bumetanide inhibition of NKCC1 activity in P31 cells repressed cisplatin-induced early-phase membrane blebbing but did not increase P31 cell resistance to cisplatin.CONCLUSIONS: Together, these results suggest that active NKCC1 was necessary for cisplatin-induced early membrane blebbing of P31 cells, but not for cisplatin-resistance. Thus, acquisition of cisplatin-resistance can affect mechanisms that have profound effects on cisplatin-induced morphological changes but are not necessary for the subsequent progression to apoptosis.
|
|
| 9. |
- Larsson, Anna-Britta, et al.
(author)
-
Klinisk slutexamination i tandhygienistprogrammet vid fyra lärosäten
- 2009
-
Reports (pop. science, debate, etc.)abstract
- Den legitimerade tandhygienisten har en nyckelroll inom promotion och prevention i svensk tandvård. I arbetet som tandhygienist krävs att kunna arbeta självständigt och ta väl underbyggda beslut för att ge en god och säker vård. Syftet med projektet var att utveckla, pröva och utvärdera en modell för klinisk slutexamination utifrån de krav som ställs för att arbeta som legitimerad tandhygienist. Projektarbetet resulterade i en modell bestående av en teoretisk och en klinisk examination. Den teoretiska examinationen består av ett fiktivt patientfall medan den kliniska examinationen omfattar en realistisk patientsituation där studenten omhändertar och behandlar en patient. Den framtagna modellens styrka är att samtliga studenter vid de olika lärosätena examineras kliniskt efter samma bedömningsgrunder vilket borgar för en god och jämförbar kvalitet.
|
|
| 10. |
- Levin, Britta, et al.
(author)
-
Memory performance during G exposure as assessed by a word recognition task
- 2007
-
In: Aviation, Space and Environmental Medicine. - 0095-6562 .- 1943-4448. ; 78:6, s. 587-592
-
Journal article (peer-reviewed)abstract
- Background: Pilots of modern fighter aircraft are exposed to substantial physiological and mental stressors. The objective of this study was to investigate how memory performance, in terms of encoding and/or retrieval processes, was affected by sustained +Gz exposure. Method: There were 18 healthy men ranging from experienced fighter pilots to novice riders who participated. A word continuous recognition task (CRT) was employed as a memory test. The task consisted of three consecutive phases: 1) encoding of familiar words at 1 G, 2) encoding and retrieval of words at 70% of the subject's relaxed G-tolerance level, equivalent to +3.7 ± 0.54 Gz, and 3) encoding and retrieval of words at 1 G. In addition, each subject performed the CRT in a 1-G-only control condition. Physiological and psycho-physiological measures included continuous monitoring of ECG, arterial oxygen saturation, arterial BP at head level, and response time. Results: Data analysis showed that the capability to recognize words encoded at 1 G did not differ between conditions, indicating that the retrieval process was insensitive to increased Gz load. However, the ability to recognize words previously encoded during G exposure was reduced by approximately 10% as compared with control. Since the analysis revealed that the words were perceived, this result suggests that the encoding process was impaired in hypergravity. Conclusion: The results indicate that memory encoding, but not retrieval, was affected negatively when exposed to substantial and sustained +Gz loads. Copyright © by Aerospace Medical Association.
|
|
