1. |
- Beal, Jacob, et al.
(author)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
2. |
|
|
3. |
|
|
4. |
|
|
5. |
- Farnocchia, Davide, et al.
(author)
-
The Second International Asteroid Warning Network Timing Campaign: 2005 LW3
- 2023
-
In: The Planetary Science Journal. - : Institute of Physics (IOP). - 2632-3338. ; 4:11
-
Journal article (peer-reviewed)abstract
- The Earth close approach of near-Earth asteroid 2005 LW3 on 2022 November 23 represented a good opportunity for a second observing campaign to test the timing accuracy of astrometric observation. With 82 participating stations, the International Asteroid Warning Network collected 1046 observations of 2005 LW3 around the time of the close approach. Compared to the previous timing campaign targeting 2019 XS, some individual observers were able to significantly improve the accuracy of their reported observation times. In particular, U.S. surveys achieved good timing performance. However, no broad, systematic improvement was achieved compared to the previous campaign, with an overall negative bias persisting among the different observers. The calibration of observing times and the mitigation of timing errors should be important future considerations for observers and orbit computers, respectively.
|
|
6. |
- Aad, G., et al.
(author)
-
- 2013
-
swepub:Mat__t (peer-reviewed)
|
|
7. |
- Aad, G., et al.
(author)
-
- 2010
-
swepub:Mat__t
|
|
8. |
- Aad, G., et al.
(author)
-
- 2011
-
swepub:Mat__t
|
|
9. |
|
|
10. |
- Aad, G., et al.
(author)
-
- 2010
-
swepub:Mat__t
|
|