| 1. |
- Abdel-Motal, Ussama M., et al.
(author)
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Major histocompatibility complex class I binding glycopeptides for the estimation of 'empty' class I molecules
- 1995
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In: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 188:1, s. 21-31
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Journal article (peer-reviewed)abstract
- Different forms of major histocompatibility complex (MHC) class I heavy chains are known to be expressed on the cell surface, including molecules which are functionally 'empty'. Direct peptide binding to cells is obvious during sensitization of target cells in vitro for cytotoxic T lymphocyte killing and 'empty' MHC-I molecules are comparatively abundant on TAP- 1 2 peptide transporter mutant cells. In the present work we have estimated the fraction of 'empty' MHC class I molecules using glycosylated peptides and cellular staining with carbohydrate specific monoclonal antibodies. Synthetic Db and Kb binding peptides were coupled at different positions with different di- or trisaccharides, using different spacing between the carbohydrate and the peptide backbone. Binding of sugar specific mAbs was compared in ELISA and cellular assays. An optimal Db binding glycopeptide was used for comparative staining with anti-Db and anti-carbohydrate monoclonal antibodies to estimate fractions of 'empty' molecules on different T lymphoid cells. On activated normal T cells, a large fraction of Db molecules were found to be 'empty'. The functional cole of such 'empty' MHC class I molecules on T cells is presently unclear. However, on antigen presenting cells they might participate in the antigen presentation process.
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| 2. |
- Adolfsson, Per I., et al.
(author)
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Characterization of α2-Adrenoceptor Subtypes in Pregnant Human Myometrium
- 1998
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In: Gynecologic and Obstetric Investigation. - : S. Karger AG. - 0378-7346 .- 1423-002X. ; 45:3, s. 145-150
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Journal article (peer-reviewed)abstract
- The aim of the present investigation was to determine which subtypes of the α2-adrenoceptors are being expressed in the human pregnant myometrium at term pregnancy. In radioligand binding studies, the specific binding of [3H]rauwolscine to human myometrial membranes was specific and of high affinity with Kd of 2.8 ± 0.6 nM and Bmax of 95 ± 5 fmol/mg protein. Results from competition for the binding of [3H]rauwolscine using subtype-selective ligands, oxymetazoline (α2A-subptype), chlorpromazine (α2B-subtype) and prazosin (α2B-α2C-subtype), suggested that the α2A- and α2B-subtypes are being co-expressed. In order to examine if also the α2C-subtype is being expressed we used an optimal concentration of oxymetazoline or chlorpromazine which would block the high-affinity site, equivalent to the α2A- and α2B-subtype respectively. Competition curves of both oxymetazoline and chlorpromazine still showed a significantly better fit using a two-site model, suggesting that the α2C-subtype also is being expressed. The expression of α2C-subtype mRNA was confirmed using reverse transcription-polymerase chain reaction on mRNA isolated from myometrial biopsies.In conclusion, our results suggest that all three subtypes of α2-adrenoceptors are being coexpressed in the human myometrium at term pregnancy and that α2-expression is dominated by the α2A-subtype.
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| 3. |
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| 4. |
- Berg, Olle H., et al.
(author)
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Comparison of observed and modeled hygroscopic behavior of atmospheric particles
- 1998
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In: Contributions to Atmospheric Physics. - 0005-8173. ; 71:1, s. 47-64
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Research review (peer-reviewed)abstract
- The hygroscopic behavior of sub-micrometer atmospheric aerosol particles was studied with a Tandem Differential Mobility Analyzer (TDMA) at a field site in the Po Valley, Italy. The measurements were done in a continental polluted aerosol during the CHEMDROP fog and haze field experiment at San Pietro di Capofiume in November 1994. In this study, hygroscopic diameter growth factors of individual particles were measured when taken from a dry state, to a relative humidity of 90 %. The aerosol consisted of two groups of particles with different hygroscopic properties, as also seen in an earlier field experiment at the same location in 1989 and at other continental sites. The present work is a closure study on the hygroscopic behavior of sub-micrometer aerosol particles and their mass. Ammonium sulfate was used to model the hygroscopic growth with a model based on thermodynamic data for non-ideal aqueous solutions at water vapor subsaturation. The study was made in two steps: The first step is a comparison between hygroscopic active aerosol volume fractions derived from TDMA measurements on individual particles integrated over the particle size distributions, and collected volume fractions of major ions sampled by size resolved cascade impactors. The model of hygroscopic growth was also used, in the second step, to calculate ambient sizes of individual aerosol particles. These sizes were then compared to the actual ambient sizes as measured by the Droplet Aerosol Analyzer. The result shows agreement, within the estimated errors, between the integrated hygroscopic active volume fractions and the collected volume fractions of inorganic salts, for five events out of six studied. A mass balance could also be obtained between the masses collected with the impactors and the integrated volume distributions, by attributing reasonable densities to the hygroscopically active and inactive fractions. The differences between the calculated and measured ambient sizes were within measurement errors, when Raoult's law was used to model the occasions with relative humidities larger than 95 %.
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| 5. |
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| 6. |
- Berg, Uno, et al.
(author)
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Sätt vid vägreparation
- 1998
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Patent (pop. science, debate, etc.)abstract
- Vid vägreparation användes ett sätt, vid vilket en vägs geometri inmätes och vägen återställes i ett önskat skick genom fräsning respektive utfyllnad av massa. Sättet innefattar kombinationen av stegen att samla in vägdata, att sammanlänka och i en databas inordna inmätta vägdata, att med hjälp av ett datorprogram projektera för återställning av vägens geometri, att skapa digital utdata från projekteringen och förse en elektronisk databärare med dessa data samt att överföra dessa data från den elektroniska databäraren till elektroniska styrandordningar för att automatiskt styra en fräsnings- och utfyllnadsprocess, som återställer vägens geometri.
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| 7. |
- Ekerfelt, Christina, 1957-, et al.
(author)
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Th2-deviation of fetus-specific T cells
- 1999
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In: Immunology today (Amsterdam. Regular ed.). - 0167-5699 .- 1355-8242. ; 20, s. 534-534
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Journal article (peer-reviewed)
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| 8. |
- Elmer, R, et al.
(author)
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K+ emission in symmetric heavy ion reactions at subthreshold energies
- 1996
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In: PHYSICAL REVIEW LETTERS. - : AMER INST PHYSICS. ; 77:24, s. 4884-4886
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Journal article (peer-reviewed)abstract
- Subthreshold K+ production cross sections have been measured in symmetric Ne + NaF, Ni + Ni, and Au + Au collisions at 1.0A GeV. The mass dependence is strong, close to A(2). The angular distributions are nonisotropic in the center-of-mass system. Introdu
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| 9. |
- Frank, Göran, et al.
(author)
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Droplet Formation and Growth in Polluted Fogs
- 1998
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In: Contributions to Atmospheric Physics. - 0005-8173. ; 71:1, s. 65-85
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Journal article (peer-reviewed)abstract
- Fog droplet formation and growth related to fog droplet activation were studied in a polluted region. The joint field experiments were carried out at San Pietro Capofiume in northern Italy during November 1994. It was found that the fog droplet number distribution was continuous in the size region 1-47 µm and that for most of the time the fog consisted of unactivated droplets, i.e. the droplets were smaller than the critical diameter for activation according to the Köhler equation. During a few time periods some of the droplets were possibly slightly larger than the critical diameter for activation. The solute concentration in the fog droplets was found to be strongly dependent and decreasing with increasing droplet size. The experimental results were compared with results obtaied using a fog model. Good overall agreement was found between the model and the experimental results, with respect to fog droplet size related to dry residue size, and to fog droplet number distribution. The fog model was also used to study the influence on fog droplet growth of the rate of temperature decrease, the aerosol particle mass load and fog liquid water content. In addition the effect of aging of the fog was also considered.
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| 10. |
- Fuzzi, Sandro, et al.
(author)
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Overview of the Po valley fog experiment 1994 (CHEMDROP)
- 1998
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In: Contributions to Atmospheric Physics. - 0005-8173. ; 71:1, s. 3-19
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Research review (peer-reviewed)abstract
- The paper presents an outline of the CHEMDROP field experiment, carried out in November 1994 at the field station of S. Pietro Capofiume in the Po Valley, Italy. The main objective of the project was to address the issue of the size-dependent chemical composition of fog droplets, by experimentally investigating the following processes, which are expected to affect (or be affected by) the chemical composition of fog droplets as a function of size: a) the connection of the size-dependent chemical composition of CCN to the size-dependent composition of fog droplets; b) the gas/liquid partitioning of the gaseous species NH3, SO2, HCHO, HNO3 in fog; c) the Fe(II)/Fe(III) redox cycle in fog water. Some general results and overall conclusions of the experiment are reported in this paper, while more specific scientific questions are discussed in other companion papers in this issue. CHEMDROP results show that several processes concur in determining the size-dependence of fog droplets chemical composition: nucleation scavenging of pre-existing CCN, fog dynamical evolution and gas/liquid exchange between interstitial air and fog droplets. Chemical transformations in the liquid phase can cause further changes in the chemical composition of the droplets. Only by taking into account the combination of all these processes, is it possible to explain the inhomogeneities in fog droplet chemical composition.
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