| 1. |
- Dutta, K., et al.
(author)
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Detection of pesticide by polymeric enzyme electrodes
- 2008
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In: Ecotoxicology and Environmental Safety. - : Elsevier Science B.V., Amsterdam. - 0147-6513 .- 1090-2414. ; 69:3, s. 556-561
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Journal article (peer-reviewed)abstract
- Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (mu g L-1). (C) 2007 Elsevier Inc. All rights reserved.
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| 2. |
- Sarkar, P., et al.
(author)
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Electrochemical immunoassay for free prostate specific antigen (f-PSA) using magnetic beads
- 2008
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In: Electroanalysis. - : John Wiley andamp;amp; Sons, Ltd. - 1040-0397 .- 1521-4109. ; 20:13, s. 1414-1420
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Journal article (peer-reviewed)abstract
- Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of less than0.1 ng mL(-1) f-PSA and a linear range of 0 to 1 ng mL(-1) f-PSA.
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