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Träfflista för sökning "WFRF:(Butler G.) srt2:(2000-2004)"

Search: WFRF:(Butler G.) > (2000-2004)

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  • Di Donna, S, et al. (author)
  • Regenerative capacity of human satellite cells : the mitotic clock in cell transplantation
  • 2000
  • In: Neurological Sciences. - New York, USA : Springer-Verlag New York. - 1590-1874 .- 1590-3478. ; 21:5 Suppl, s. S943-51
  • Journal article (peer-reviewed)abstract
    • In this communication, we will review the problems caused by cell-mediated gene therapy, taking skeletal muscle as a physiological model. In particular we have utilised vectors transferring telomerase under the control of retroviral promoters into human satellite cells. The set of results presented here has several implications regarding gene therapy trials. Nevertheless, more experiments will be required to fully validate this cellular model and to use telomerase to safely extend the lifespan of putative gene therapy vectors.
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  • Oldmixon, E. H., et al. (author)
  • alpha-Actin : disposition, quantities, and estimated effects on lung recoil and compliance
  • 2001
  • In: Journal of applied physiology. - : American Physiological Society. - 8750-7587 .- 1522-1601. ; 91:1, s. 459-473
  • Journal article (peer-reviewed)abstract
    • We have investigated the basis and implications of pneumoconstriction by measuring disposition and quantities of alpha -smooth muscle actin in rat and guinea pig lungs and modeling its effects on lung recoil and compliance. A robust marker of contractility, alpha -smooth muscle actin appears in smooth muscle or myofibroblast-like cells in pleura, airways, blood vessels, and alveolar ductal tissues. In each site, we measured its transected area by immunofluorescent staining and frequency-modulated scanning confocal microscopy. We incorporated these data in a model of the parenchyma consisting of an extensive elastic network with embedded contractile structures. We conclude that contraction at any one of these sites alone can decrease parenchymal compliance by 20-30% during tidal breathing. This is due mostly to the stiffness of activated contractile elements undergoing passive cycling; constant muscle tension would have little effect. The magnitude of the effect corresponds with known responses of the lung to hypocapnia, consistent with a homeostatic function in which gas exchange is defended by redistributing ventilation away from overventilated units.
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