| 1. |
- Botusan, I. R., et al.
(author)
-
Deficiency of liver-derived insulin-like growth factor-I (IGF-I) does not interfere with the skin wound healing rate
- 2018
-
In: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 13:3
-
Journal article (peer-reviewed)abstract
- Objective: IGF-I is a growth factor, which is expressed in virtually all tissues. The circulating IGF-I is however derived mainly from the liver. IGF-I promotes wound healing and its levels are decreased in wounds with low regenerative potential such as diabetic wounds. However, the contribution of circulating IGF-I to wound healing is unknown. Here we investigated the role of systemic IGF-I on wound healing rate in mice with deficiency of liver-derived IGF-I (LI-IGF-I-/- mice) during normal (normoglycemic) and impaired wound healing (diabetes). Methods: LI-IGF-I-/- mice with complete inactivation of the IGF-I gene in the hepatocytes were generated using the Cre/loxP recombination system. This resulted in a 75% reduction of circulating IGF-I. Diabetes was induced with streptozocin in both LI-IGF-I-/- and control mice. Wounds were made on the dorsum of the mice, and the wound healing rate and histology were evaluated. Serum IGF-I and GH were measured by RIA and ELISA respectively. The expression of IGF-I, IGF-II and the IGF-I receptor in the skin were evaluated by qRT-PCR. The local IGF-I protein expression in different cell types of the wounds during wound healing process was analyzed using immunohistochemistry. Results: The wound healing rate was similar in LI-IGF-I-/- mice to that in controls. Diabetes significantly delayed the wound healing rate in both LI-IGF-I-/- and control mice. However, no significant difference was observed between diabetic animals with normal or reduced hepatic IGF-I production. The gene expression of IGF-I, IGF-II and IGF-I receptor in skin was not different between any group of animals tested. Local IGF-I levels in the wounds were similar between of LI-IGF-I-/- and WT mice although a transient reduction of IGF-I expression in leukocytes in the wounds of LI-IGF-I-/- was observed seven days post wounding. Conclusion: Deficiency in the liver-derived IGF-I does not affect wound healing in mice, neither in normo-glycemic conditions nor in diabetes.
|
|
| 2. |
- Asensio-Torres, Ruben, et al.
(author)
-
SPOTS : The Search for Planets Orbiting Two Stars III. Complete sample and statistical analysis
- 2018
-
In: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 619
-
Journal article (peer-reviewed)abstract
- Binary stars constitute a large percentage of the stellar population, yet relatively little is known about the planetary systems orbiting them. Most constraints on circumbinary planets (CBPs) so far come from transit observations with the Kepler telescope, which is sensitive to close-in exoplanets but does not constrain planets on wider orbits. However, with continuous developments in high-contrast imaging techniques, this population can now be addressed through direct imaging. We present the full survey results of the Search for Planets Orbiting Two Stars (SPOTS) survey, which is the first direct imaging survey targeting CBPs. The SPOTS observational program comprises 62 tight binaries that are young and nearby, and thus suitable for direct imaging studies, with VLT/NaCo and VLT/SPHERE. Results from SPOTS include the resolved circumbinary disk around AK Sco, the discovery of a low-mass stellar companion in a triple packed system, the relative astrometry of up to 9 resolved binaries, and possible indications of non-background planetary-mass candidates around HIP 77911. We did not find any CBP within 300 AU, which implies a frequency upper limit on CBPs (1-15 M-Jup) of 6-10% between 30-300 AU. Coupling these observations with an archival dataset for a total of 163 stellar pairs, we find a best-fit CBP frequency of 1.9% (2-15 M-Jup) between 1 and 300 AU with a 10.5% upper limit at a 95% confidence level. This result is consistent with the distribution of companions around single stars.
|
|
