| 1. |
- Alvarez, Maria Teresa, et al.
(author)
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Lab-scale production of biogenic sulphide for metal precipitation in remote areas
- 2012
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In: International Journal of Environment and Waste Management. - 1478-9876. ; 9:3-4, s. 313-329
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Journal article (peer-reviewed)abstract
- Batch cultures with wheat straw, biomass of Paja Brava (Festuca orthophylla), filter paper, newspaper and beech leaves (Fagus sylvatica) were established to produce sulphide. Sulphide production, sulphate reduction, concentration of Volatile Fatty Acids (VFAs), enzyme activities and Fluorescence in situ hybridisation were determined. Approximately 5 mM of sulphide was produced during anaerobic digestion of wheat straw, while the production with newspaper as carbon source was the lowest (ca.1 mM). The sulphide production (2-5 mM) in the semi-continuous culture of the consortium A10, using wheat straw supported Cu(II), Pb (II) and Zn (II) removal up to 90%.
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| 2. |
- Linares-Pastén, Javier, et al.
(author)
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A method for rapid screening of ketone biotransformations: Detection of whole cell Baeyer-Villiger monooxygenase activity.
- 2012
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In: Enzyme and Microbial Technology. - : Elsevier BV. - 0141-0229. ; 50:2, s. 101-106
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Journal article (peer-reviewed)abstract
- A method for screening of ketone biotransformations was developed and applied to the identification of Baeyer-Villiger monooxygenase (BVMO) activity. The method was based on the formation of a purple coloured product on reaction between an enolizable ketone and 3,5-dinitrobenzoic acid in an alkaline solution. Absorbance of the colour decreased with the size of the cycloketone ring. Stoichiometric ratio between cycloketone and 3,5-dinitrobenzoic acid was 1:1 at maximum absorbance. The method was applied for monitoring the consumption of cyclohexanone by bacteria under aerobic conditions, and was found to be potentially useful for both screening assays and quantitative measurements of BVMO activity. Compared to other existing methods, this method is faster, cheaper and amenable for whole cell assays.
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