| 1. |
|
|
| 2. |
- Ekholm, S. E., et al.
(author)
-
MRI staging using gadodiamide for soft-tissue tumors of the head and neck region. Results from a phase II trial and a 5-year clinical follow-up
- 2001
-
In: Eur J Radiol. - 0720-048X. ; 39:3, s. 168-75
-
Journal article (peer-reviewed)abstract
- In order to document the safety, tolerability and efficacy of gadodiamide outside CNS, an open, non-drug comparative study was performed in patients with tumors of the head and neck region. Fifty adult patients were included and 48 patients received the contrast medium. The examinations were performed on a 1.5 T imager using transverse, non-enhanced T1- and PD-/T2-weighted conventional spin-echo sequences, followed by a contrast-enhanced transverse T1-weighted sequence. Post-contrast images provided more diagnostic information compared to unenhanced images in 33 of 48 patients (69%). This information was of significant help in four and of moderate help in 14 cases. Post-contrast images compared to non-enhanced T1-weighted showed improvement in lesion delineation for 29 of the 43 patients where a lesion was observed. Only in two patients was the diagnostic information lower post-contrast. A comparison between all pre-contrast images versus contrast medium enhanced showed post-contrast images to give more diagnostic information in 14 and less in nine patients. No patient experienced discomfort in relation to gadodiamide injection. Only one adverse event occurred which was described as thirst, being of moderate intensity. The 5-year clinical outcome was analyzed and compared with the pre-operative staging. The case-books of all patients were reviewed and in 44 patients all information could be found. Of those, 18 were still alive, one with active disease (AAD) and 17 with no evidence of disease (NED). Two of those four patients, where information was incomplete, showed NED and two had died. This trial showed that contrast-enhancement using gadodiamide for evaluation of soft tissue tumors in the head and neck region was safe and provided statistically significant more diagnostic information compared with unenhanced images. MRI, when compared with palpation/inspection, changed tumor staging in approximately 30% of all cases.
|
|
| 3. |
- Williams, N M, et al.
(author)
-
A systematic genomewide linkage study in 353 sib pairs with schizophrenia.
- 2003
-
In: American Journal of Human Genetics. - : Elsevier BV. - 0002-9297 .- 1537-6605. ; 73:6, s. 1355-1367
-
Journal article (peer-reviewed)abstract
- We undertook a genomewide linkage study in a total of 353 affected sib pairs (ASPs) with schizophrenia. Our sample consisted of 179 ASPs from the United Kingdom, 134 from Sweden, and 40 from the United States. We typed 372 microsatellite markers at approximately 10-cM intervals. Our strongest finding was a LOD score of 3.87 on chromosome 10q25.3-q26.3, with positive results being contributed by all three samples and a LOD-1 interval of 15 cM. This finding achieved genomewide significance (P<.05), on the basis of simulation studies. We also found two regions, 17p11.2-q25.1 (maximum LOD score [MLS] = 3.35) and 22q11 (MLS = 2.29), in which the evidence for linkage was highly suggestive. Linkage to all of these regions has been supported by other studies. Moreover, we found strong evidence for linkage (genomewide P<.02) to 17p11.2-q25.1 in a single pedigree with schizophrenia. In our view, the evidence is now sufficiently compelling to undertake detailed mapping studies of these three regions.
|
|
| 4. |
- Choi, Young-Hun, et al.
(author)
-
Identification of a novel isoform of the cyclic-nucleotide phosphodiesterase PDE3A expressed in vascular smooth-muscle myocytes
- 2001
-
In: Biochemical Journal. - 0264-6021. ; 353:Pt 1, s. 41-50
-
Journal article (peer-reviewed)abstract
- We have identified a new cyclic-nucleotide phosphodiesterase isoform, PDE3A, and cloned its cDNA from cultured aortic myocytes. The nucleotide sequence of its coding region is similar to that of the previously cloned myocardial isoform except for the absence of the initial 300-400 nt that are present in the latter, as confirmed by reverse-transcriptase-mediated PCR, 5' rapid amplification of cDNA ends and a ribonuclease protection assay. Expression in Spodoptera frugiperda (Sf9) cells yields a protein with catalytic activity and inhibitor sensitivity typical of the PDE3 family. The recombinant protein's molecular mass of approx. 131 kDa is compatible with translation from an ATG sequence corresponding to nt 436-438 of the myocardial PDE3A coding region. Antibodies against residues 424-460 (nt 1270-1380) and 1125-1141 (nt 3373-3423) of the myocardial isoform react with an approx. 118 kDa band in Western blots of homogenates of human aortic myocytes, whereas antibodies against residues 29-42 (nt 85-126) do not react with any bands in these homogenates. Our results suggest that a vascular smooth-muscle isoform ('PDE3A2') is a product of the same gene as the longer myocardial ('PDE3A1') and the shorter placental ('PDE3A3') isoforms and is generated pre-translationally in a manner that results in the absence of the 145 N-terminal amino acids of PDE3A1.
|
|
| 5. |
- Danielsson, Aina, 1953, et al.
(author)
-
The prevalence of disc aging and back pain after fusion extending into the lower lumbar spine. A matched MR study twenty-five years after surgery for adolescent idiopathic scoliosis.
- 2001
-
In: Acta radiologica (Stockholm, Sweden : 1987). - 0284-1851. ; 42:2, s. 187-97
-
Journal article (peer-reviewed)abstract
- To determine the long-term outcome after fusion for adolescent idiopathic scoliosis in terms of degenerative disc findings diagnosed using MR imaging and to elucidate the clinical consequences.Thirty-two patients with adolescent idiopathic scoliosis, who had undergone spinal fusion using Harrington rods to the lower lumbar spine with one or two unfused discs below the fusion, were re-examined 25 years after the fusion. The re-examinations included validated questionnaires, clinical examination, full standing frontal and lateral radiographs and MR examination of the lower lumbar region. Curve size and degenerative findings on MR images were evaluated by two unbiased radiologists, blinded to the clinical findings. A matched control group of 32 persons without scoliosis was subjected to the same examinations.There were significantly more degenerative disc changes (p<0.0001), disc height reduction (p=0.0010) and end-plate changes (p<0.0001 for both upper and lower end-plates) in the lowest unfused disc in the patient group compared with the control group. The MR findings in the lowest unfused disc, but not the one above, in the patient group correlated to lumbar pain intensity as well as to the diminished lumbar lordosis.
|
|
| 6. |
- Eir, A., et al.
(author)
-
From rough to final designs by incremental set-inclusion of properties
- 2002
-
Conference paper (peer-reviewed)abstract
- Design of buildings is a complex task in which ideas are sketched and communicated, by representations that are incrementally elaborated from the early rough sketches to the final design. We claim, that today’s model–based design tools are restricted from fully supporting this process as they are founded on the principle that objects are instances of static types. Such systems do not offer work with objects being incrementally specialised according to their properties, and neither do they offer dynamics of the underlying type system.The present paper elaborates on a property–oriented approach as a foundation for design tools facilitating incremental design based on set–inclusion of properties. We emphasize the formal foundation for incorporating such dynamics, and we specify requirements for tools facilitating incremental design and offering improved semantic support.
|
|
| 7. |
- Ekholm, P., et al.
(author)
-
A quartz crystal microbalance study of the adsorption of asphaltenes and resins onto a hydrophilic surface
- 2002
-
In: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797 .- 1095-7103. ; 247:2, s. 342-350
-
Journal article (peer-reviewed)abstract
- The adsorption of extracted and purified samples of asphaltenes and resins onto gold surfaces has been studied as a function of bulk concentration using a quarts crystal microbalance with dissipation measurements (QCM-D). With this device, which works equally well in transparent, opaque, and nontransparent samples, the adsorbed amount is measured through a change in resonant frequency of the quartz oscillator. The measured change in dissipation reports on changes in layer viscoelasticity and slip of the solvent at the surface. The results show that the adsorbed amount for resins from heptane corresponds to a rigidly attached monolayer. The adsorbed amount decreases with increasing amount of toluene in the solvent and is virtually zero in pure toluene. Asphaltenes, on the other hand, adsorb in large quantities and the mass and dissipation data demonstrate the presence of aggregates on the surface. The aggregates are firmly attached and cannot be removed by addition of resins. On the other hand, resins and asphaltenes associate in bulk liquid and the adsorption from mixtures containing both resins and asphaltenes is markedly different from that obtained from the pure components. Hence, we conclude that preformed resin aggregates adsorb to the surface. These results are compared and discussed in relation to adsorption from crude oil diluted in heptane/toluene mixtures.
|
|
| 8. |
- Ekholm-Reed, S, et al.
(author)
-
Deregulation of cyclin E in human cells interferes with prereplication complex assembly
- 2004
-
In: The Journal of cell biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 165:6, s. 789-800
-
Journal article (peer-reviewed)abstract
- Deregulation of cyclin E expression has been associated with a broad spectrum of human malignancies. Analysis of DNA replication in cells constitutively expressing cyclin E at levels similar to those observed in a subset of tumor-derived cell lines indicates that initiation of replication and possibly fork movement are severely impaired. Such cells show a specific defect in loading of initiator proteins Mcm4, Mcm7, and to a lesser degree, Mcm2 onto chromatin during telophase and early G1 when Mcm2–7 are normally recruited to license origins of replication. Because minichromosome maintenance complex proteins are thought to function as a heterohexamer, loading of Mcm2-, Mcm4-, and Mcm7-depleted complexes is likely to underlie the S phase defects observed in cyclin E–deregulated cells, consistent with a role for minichromosome maintenance complex proteins in initiation of replication and fork movement. Cyclin E–mediated impairment of DNA replication provides a potential mechanism for chromosome instability observed as a consequence of cyclin E deregulation.
|
|
| 9. |
|
|
| 10. |
|
|
