| 1. |
- Lindskog, Sven, et al.
(author)
-
Validation of an Algorithm for Chronic Periodontitis Risk Assessment and Prognostication : Analysis of an Inflammatory Reactivity Test and Selected Risk Predictors
- 2010
-
In: Journal of Periodontology. - : American Academy of Periodontology. - 0022-3492 .- 1943-3670. ; 81:6, s. 837-847
-
Journal article (peer-reviewed)abstract
- Background: Patients with severe forms of chronic periodontitis present with varying degrees of decreased inflammatory reactivity. A previously reported algorithm for chronic periodontitis risk assessment and prognostication is based on the analysis of some 20 risk predictors. One of these predictors is a skin provocation test that assesses the individual patient's reactivity to a lipid A challenge. The aim of this report was to analyze results from validation data for the algorithm with respect to the contribution of results of the skin provocation test as a risk predictor for the progression of chronic periodontitis and to compare these results with the contribution from other predictors, namely smoking, angular bony destruction, furcation involvement, abutment teeth, and endodontic pathology. Methods: Data from a previously reported clinical validation sample were used for the analysis, including the calculation of quality measures and explanatory values using different types of regression analysis and non-parametric testing. Results: Smoking, endodontic pathology, abutment teeth, angular bony destruction, and furcation involvement presented with individual explanatory values for periodontitis progression between 4% and 13% and highly significant parameter estimates. Explanatory values for the results of the skin provocation test ranged between 2.6% and 5.1% depending on the disease severity group, with a positive predictive value of 82% for the identification of high-risk patients. Conclusion: The skin provocation test provided a clinically significant contribution to the quality of analysis with the periodontitis risk and prognostication algorithm, in particular in the selection of high-risk patients for in-depth individual tooth analysis.
|
|
| 2. |
- Lindskog, Sven, et al.
(author)
-
Validation of an Algorithm for Chronic Periodontitis Risk Assessment and Prognostication: Risk Predictors, Explanatory Values, Measures of Quality, and Clinical Use
- 2010
-
In: JOURNAL OF PERIODONTOLOGY. - : American Academy of Periodontology. - 0022-3492 .- 1943-3670. ; 81:4, s. 584-593
-
Journal article (peer-reviewed)abstract
- Background: The American Academy of Periodontology has recently stated that, "[risk assessment will become] increasingly important in periodontal treatment planning and should be part of every comprehensive dental and periodontal evaluation." (J Periodontol 2006;77:1608). Unaided risk assessment and prognostication show significant variability because chronic periodontitis is a multifactorial disease. This report summarizes the clinical validation of an algorithm for chronic periodontitis risk assessment and prognostication. The algorithm is a Web-based analytic tool that integrates some 20 risk predictors and calculates scores indicating levels of risk for chronic periodontitis for the dentition (Level I) and, if an elevated risk is found, prognosticates disease progression tooth by tooth (Level II). Methods: An independent clinical validation sample was generated in an open, prospective clinical trial and analyzed in a predetermined validation plan. Results: The analyses identified two threshold scores above which significant progression of periodontitis was found. Based on these scores, sufficiently high explanatory values with significant and increasing parameter estimates for increasing risk were established in Level I, justifying detailed analysis tooth by tooth in Level II. Subsequent prognostication of chronic periodontitis in Level II was found to be accompanied by clinically relevant measures of quality in relation to rates of disease progression. Three score intervals representing increasing levels of periodontitis progression were identified corresponding to increasing levels of significant annual marginal bone loss. Conclusions: The predictors included in the algorithm reflect a relevant selection for periodontitis risk assessment. Risk assessment and prognostication with the algorithm provides the clinician with a validated, reliable, consistent, and objective tool supporting treatment planning.
|
|
| 3. |
- Ericsson, Claes, 1947-, et al.
(author)
-
Marknadsestetik och vardagskultur i klassrummet : Ett ideologiskt dilemma? Identitet, dominans och kunskapsbildning i grundskolans musikundervisning
- 2010
-
In: Resultatdialog 2010. - Stockholm : Vetenskapsrådet. - 9789173071840 ; , s. 42-50
-
Book chapter (peer-reviewed)abstract
- De senaste decennierna har successivt en medvetenhet och insikt om att skolan inte har monopol på kunskapsbildning växt fram. Skolan konkurrensutsätts i ständigt ökande utsträckning från andra kunskapsbildningsinstanser, exempelvis via sofistikerad informations- och kommunikationsteknologi, men även från institutioner som bildningsförbund och föreningar av olika slag. Detta tillsammans med en mera allmän tendens i samhället att nedmontera traditioner och auktoritetsförhållanden skapar möjlighet för elever att i högre utsträckning än tidigare ifrågasätta skolan med utgångspunkt i ett vetande som är tillägnat utanför skolan. En av de verksamheter som i hög grad berörs är den som bedrivs inom ramen för de så kallade estetiska ämnena. Detta för att barn och ungdomar har ett gediget kunnande och en distinkt preferens inom området, som de tillägnar sig i ofta informella lärsituationer i vardagslivet, parallellt med lärandet i skolan. Ett antagande är därvid att de estetiska ämnena kan uppfattas som ”mer än skolämnen”, vilket kan innebära en distorsion mellan skolans och elevernas förväntningar på dessa ämnen. Detta bekräftas av två av våra tidigare studier. Syftet med studien är: 1. Att undersöka vilka dominansförhållanden och identitetsförhandlingar som kommer till uttryck när elevers vardagskultur omsätts i skolan. 2. Att beskriva kunskapsbildningens karaktär i undervisningskontexter präglade av skilda strukturella förutsättningar, exempelvis sociala, kulturella, ekonomiska, utbildningsmässiga samt demografiska. Vid tre skolor, där skilda bakgrundsförutsättningar råder, kommer undervisningen i musik att dokumenteras med hjälp av videokamera. En undervisningsgrupp (år 9) vid varje skola kommer att väljas ut och följas kontinuerligt under ett läsår. Interaktionen mellan lärare och elever och mellan elever sinsemellan kommer att analyseras med avseende på verbal och visuell kommunikation. Resultaten kommer att diskuteras med utgångspunkt i ett samhälls- och kulturteoretiskt perspektiv. Projektet är tänkt att löpa under tre år och ny kunskap bör kunna avsättas under 2009. Att studera makt- och dominansförhållanden i skolan får ses som angeläget. Dels kan det initiera till reflektion, vilket i en förlängning kan leda till förändring inom de pedagogiska praktikerna, dels är det ett led i kvalitetssäkringen av skolan. Att studera identitetsförhandling i mötet mellan elevers vardagskultur och skola är också betydelsefullt, eftersom skolans fostransuppdrag kan komma i konflikt med den strävan som finns att bejaka ungdomars identitetsbildning. Det är viktigt att dessa i systemet ingående motsättningar belyses, inte minst med avseende på genusproblematik. Att studera kunskapsbildningens karaktär i pedagogiska kontexter där bakgrundsförutsättningarna varierar, framstår också som angeläget. Sådan forskning kan ses som betydelsefull både för realiserandet av en likvärdig skola och som diskussionsunderlag vid utarbetande av olika typer av undervisningsreglerande texter. Forskningsspridningen kommer att ske på olika sätt. Rapporten ges ut i bokform, vilket ökar tillgängligheten. Resultaten kommer också att kommuniceras via seminarier på skolor och lärarutbildningar. Slutligen kommer resultaten att återföras till de i undersökningen deltagande skolorna, där lärarna kommer att beredas möjlighet till reflektion i diskussionsgrupper, exempelvis i anslutning till studiedagar
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Ericsson, Claes, 1947-, et al.
(author)
-
The Music Classroom in Focus : Everyday Culture, Identity, Governance and Knowledge Formation
- 2010
-
In: Nordic Research in Music Education. - Oslo : Norges musikkhøgskole. - 0333-3760. ; 12, s. 101-116
-
Journal article (peer-reviewed)abstract
- This article is based on a larger research project with the purpose to study how market aesthetics and student’s music culture are expressed in the Swedish music classroom. The empirical material consists of video observations of classroom activities in secondary school settings in Sweden. The theoretical framework consists of poststructuralist and social constructionist theory combined with theories of late modernity, while our methodological point of departure is discourse analysis. Some important analytical concepts are identity, dominance, governance and knowledge formation. Three different strategies for incorporating market aesthetics and students’ music culture into music education were identified: learning about, reflecting on and applying. An ideological dilemma occurred when the fostering mission of school was confronted with the will to meet the students’ demands for freedom of expression. The results of the project also suggest that standardised and regulated forms of activity were counterproductive to creativity in music making. Six different strategies of gentle governance in the music classroom were identified. Popular music was presented by the teachers in a way analogous to the canon of art music that is predominant in the teaching of music history at school.
|
|
| 8. |
- Ericsson, Claes, 1947, et al.
(author)
-
The music classroom in focus. Everyday culture, identity, governance and knowledge formation.
- 2010
-
In: Nordic Research in Music Education. Yearbook. - 1504-5021. ; 12, s. 101-116
-
Journal article (peer-reviewed)abstract
- This article is based on a larger research project with the purpose to study how market aesthetics and student’s music culture are expressed in the Swedish music classroom. The empirical material consists of video observations of classroom activities in secondary school settings in Sweden. The theoretical framework consists of poststructuralist and social constructionist theory combined with theories of late modernity, while our methodological point of departure is discourse analysis. Some important analytical concepts are identity, dominance, governance and knowledge formation. Three different strategies for incorporating market aesthetics and students’ music culture into music education were identified: learning about, reflecting on and applying. An ideological dilemma occurred when the fostering mission of school was confronted with the will to meet the students’ demands for freedom of expression. The results of the project also suggest that standardised and regulated forms of activity were counterproductive to creativity in music making. Six different strategies of gentle governance in the music classroom were identified. Popular music was presented by the teachers in a way analogous to the canon of art music that is predominant in the teaching of music history at school. Keywords: Music classroom, market aesthetics, everyday culture, discourse, identity, music education
|
|
| 9. |
- Ericsson, Emma, 1981-, et al.
(author)
-
Controlled Orientation and Covalent Attachment of Proteins on Biosensor Surfaces by Chelation Assisted Photoimmobilization
- 2013
-
Other publication (other academic/artistic)abstract
- This report presents a novel method for uniform orientation and covalent attachment of proteins to sensing surfaces, termed Chelation Assisted Photoimmobilization (CAP). Alkanethiols terminated with either nitrilotriacetic acid (NTA), benzophenone (BP) or oligo(ethylene glycol) were synthesized and mixed self-assembled monolayers (SAMs) were prepared on gold and thoroughly characterized by infrared reflection absorption spectroscopy (IRAS), ellipsometry and contact angle goniometry. In the process of CAP, NTA chelates Ni2+ and the complex coordinates a His-tagged ligand in an oriented assembly. The ligand is then photoimmobilized via BP, which forms covalent bonds upon UV light activation. The CAP concept was demonstrated using human IgG-Fc modified with C-terminal hexahistidine tags (His-IgGFc) as the ligand and protein A as the analyte.In the development of affinity biosensors, uniform orientation of ligand molecules where all analyte binding sites are accessible is often preferred to random orientation. In order to monitor the effect of ligand orientation on analyte response, the ligand-analyte interaction was quantified by surface plasmon resonance analysis, both in the case of CAP and when the ligand was attached by conventional amine coupling on surfaces presenting NTA. Responses were adjusted for differences in ligand immobilization level using IRAS. The normalized analyte response with randomly oriented ligand was 2.5 times higher than that with ligand immobilized by CAP, probably due to molecular crowding effects on the surface and the fact that His-IgGFc is bivalent for protein A. This is a reminder that many other factors than orientation alone may play a decisive role in analyte binding on biosensor surfaces.
|
|
| 10. |
- Ericsson, Emma M, et al.
(author)
-
Controlled orientation and covalent attachment of proteins on biosensor surfaces by Chelation Assisted Photoimmobilization
- 2013
-
Conference paper (other academic/artistic)abstract
- In the context of surface chemistry for affinity biosensor chips, it is widely accepted that uniform orientation of the immobilized recognition element (ligand) is preferred over random orientation. However, this assumption has often been based on studies where differences in ligand immobilization level have not been taken into account. In this contribution, we present a novel two-step method for homogenous orientation and covalent attachment of proteins to sensing surfaces, called Chelation Assisted Photoimmobilization (CAP). Careful quantification of the effect of ligand orientation on analyte responses was performed by comparing this strategy to immobilization by conventional amine coupling. In CAP, the chelation agent is nitrilotriacetic acid (NTA) which chelates Ni2+. A His-tagged ligand forms an oriented assembly when binding Ni2+-NTA and is then covalently bound to the surface via photolabile benzophenone (BP), which attacks C-H bonds upon UV light activation. We relied on a surface chemistry based on self-assembled monolayers (SAMs) of oligo(ethylene glycol) (OEG)-containing alkanethiolates on gold. Alkanethiols terminated with either NTA, BP or OEG were synthesized and mixed SAMs were characterized by infrared reflection absorption spectroscopy (IRAS), ellipsometry and contact angle goniometry. IRAS was also used to quantify ligand immobilization levels obtained either by CAP or by amine coupling via the carboxyl groups of an NTA-presenting surface. The model ligand was human IgG-Fc modified with a C-terminal 6xHis-tag and the analyte was Protein A. The ligand-analyte interaction was quantified by a surface plasmon resonance biosensor. Analyte responses were normalized with respect to the ligand amounts obtained by the two immobilization strategies. Interestingly, the normalized analyte response with randomly oriented ligand was >2 times higher than that with ligand immobilized by CAP. This shows that oriented ligand immobilization is not necessarily a means of increasing the sensitivity of a biosensor. Factors that may influence performance include the valency of the ligand and constraints related to the surface chemistry used for orientation.
|
|
