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Träfflista för sökning "WFRF:(Fernandez E.) srt2:(1985-1989)"

Search: WFRF:(Fernandez E.) > (1985-1989)

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1.
  • Fernandez-Luna, J L, et al. (author)
  • A sensitive and rapid enzyme-linked immunosorbent assay using monoclonal antibodies for simultaneous quantitation of free and IgA-complexed protein HC
  • 1985
  • In: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 82:1, s. 101-110
  • Journal article (peer-reviewed)abstract
    • A sensitive and rapid enzyme-linked immunosorbent assay for simultaneous quantitation of free and IgA-complexed human protein HC was developed with monoclonal murine antibodies. The total amount of protein HC (free plus IgA-complexed) was measured by a competitive procedure while the protein HC-IgA complex was quantitated by a sandwich enzyme immunoassay. The amount of free protein HC was then obtained as the difference between the 2 measured values. The sensitivity of the procedure was 70 micrograms/1 for the total amount of protein HC and 80 micrograms/1 for the protein HC-IgA complex. At ordinary human serum levels of free protein HC and protein HC-IgA complexes the coefficient of variation for the procedure was about 5%. One worker could determine the concentrations of free and IgA-complexed protein HC in up to 400 serum samples in a working day.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Grubb, Anders (1)
Grubb, A (1)
Méndez, E (1)
Lopez, Carlos (1)
Méndez, Enrique (1)
Fernandez-Luna, J L (1)
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Moneo, Ignacio (1)
Fernandez-Luna, JL (1)
Mihaesco, E (1)
Vaerman, J-P (1)
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University
Lund University (2)
Language
English (2)
Research subject (UKÄ/SCB)
Medical and Health Sciences (2)

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