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- Blomqvist, M, et al.
(author)
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Identification of defensins in human lymphocyte nuclei
- 1999
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In: EUROPEAN JOURNAL OF BIOCHEMISTRY. - : BLACKWELL SCIENCE LTD. - 0014-2956. ; 263:2, s. 312-318
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Journal article (other academic/artistic)abstract
- The cell nucleus plays an essential role in all aspects of cell function and regulation. Most of the nuclear proteins/ peptides are synthesized in the cytoplasm. and transported into the nucleus through the nuclear pore complexes. The nuclear proteins/pep
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2. |
- Blomqvist, M, et al.
(author)
-
Identification of defensins in human lymphocyte nuclei
- 1999
-
In: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 263:2, s. 312-8
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Journal article (peer-reviewed)abstract
- The cell nucleus plays an essential role in all aspects of cell function and regulation. Most of the nuclear proteins/peptides are synthesized in the cytoplasm and transported into the nucleus through the nuclear pore complexes. The nuclear proteins/peptides conjugate with each other and interact in transcriptional activation/inactivation. Several of the high molecular mass transcription factors (> 30 kDa) have been identified and characterized. However, the information on the low molecular mass proteins/peptides of the nucleus is limited. We have investigated these low molecular mass proteins/peptides from the nucleus of human peripheral blood lymphocytes using reversed-phase high-performance liquid chromatography (RP-HPLC). The HPLC fractions were further analysed by matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, electrospray ionization time of flight (ESI-TOF) mass spectrometry and electrospray ionization fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry for mass determination. Using this combination of mass spectrometry techniques and microsequence analysis, we have shown that human lymphocyte nuclei contain defensins, a mixture of human neutrophil granule peptide 1, 2 and 3.
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