| 1. |
- Funk, C, et al.
(author)
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Influence of Growth Regulators and an Elicitor on Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia
- 1990
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In: Phytochemistry. - 0031-9422 .- 1873-3700. ; 29:3, s. 845-848
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Journal article (peer-reviewed)abstract
- A cell suspension culture of Vanilla planifolia has been established in MS-medium. 2,4-D suppressed while NAA enhanced the formation of extractable phenolics. Cytokinins appeared to favour lignin biosynthesis. Treatment of the culture with chitosan resulted in the induction of various enzymes of phenylpropanoid metabolism, while the amount of extractable phenolics decreased due to their rapid incorporation into polymeric ligneous material.
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| 2. |
- Funk, C, et al.
(author)
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Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia. Andr. II. Effects of Precursor Feeding and Metabolic Inhibitors
- 1990
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In: Plant Physiology. - 0032-0889 .- 1532-2548. ; 94, s. 95-101
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Journal article (peer-reviewed)abstract
- Feeding of cinnamic acid and ferulic acid to non-treated andchitosan-treated cell suspension cultures of Vanilla planifoIiaresulted in the formation of trace amounts of p-hydroxy benzoicacid (5.2 micrograms per gram fresh weight of cells) and vanillicacid (6.4 micrograms per gram fresh weight of cells), respectively.Addition of a 4-hydroxycinnamate: CoA-ligase inhibitor, 3,4-(methylenedioxy)-cinnamic acid (MDCA), resulted in a reducedbiosynthesis of ligneous material with a simultaneous significantincreased vanillic acid formation (around 75 micrograms per gramfresh weight of cells). A K, of 100 micromolar for 4-hydroxycinnamate:CoA-ligase in a crude preparation was estimated for thisinhibitor. It is suggested that the conversion of cinnamic acidsinto benzoic acids does not involve cinnamoyl CoA esters asintermediates. Feeding of 14C-cinnamic acid and 14C-ferulic acidto cells treated with MDCA indicate that cinnamic acid, but notferulic acid, is a precursor of vanillic acid in these cultivated cellsof V. planifolia.
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| 3. |
- Funk, C, et al.
(author)
-
Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia. Andr. III. Conversion of 4-Methoxycinnamic acids into 4-Hydroxybenzoic acids
- 1990
-
In: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 94, s. 102-108
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Journal article (peer-reviewed)abstract
- Feeding of 4-methoxycinnamic acid, 3,4-dimethoxycinnamicacid and 3,4,5-trimethoxycinnamic acid to cell suspension culturesof Vanilla planifolia resulted in the formation of 4-hydroxybenzoicacid, vanillic acid, and syringic acid, respectively. Thehomologous 4-methoxybenzoic acids were demethylated to thesame products. It is concluded that the side chain degradingenzyme system accepts the 4-methoxylated substrates while thedemethylation occurs at the benzoic acid level. The demethylatingenzyme is specific for the 4-position. Feeding of [10_4Cmethyl]-3,4-dimethoxycinnamic acid revealed that the first stepin the conversion is the glycosylation of the cinnamic acid to itsglucose ester. A partial purification of a UDP-glucose: transcinnamicacid glucosyltransferase is reported. 4-Methoxy substitutedcinnamic acids are better substrates for this enzyme than4-hydroxy substituted cinnamic acid. It is suggested that 4-methoxysubstituted cinnamic acids are intermediates in the biosyntheticconversion of cinnamic acids to benzoic acids in cells ofV. planifolia.
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| 4. |
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