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| 2. |
- Denmeade, Samuel R., et al.
(author)
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Enzymatic activation of a doxorubicin-peptide prodrug by prostate- specific antigen
- 1998
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In: Cancer Research. - 0008-5472. ; 58:12, s. 2537-2540
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Journal article (peer-reviewed)abstract
- New approaches to target cytotoxic therapy specifically to metastatic prostate cancer sites are urgently needed. As such an approach, an inactive prodrug was synthesized by coupling the primary amine of doxorubicin to the COOH-terminal carboxyl of a seven-amino acid peptide carrier (i.e., Mu-His- Ser-Ser-Lys-Leu-Gln-Leu). The seven-amino acid peptide was documented to be hydrolyzable specifically by the serine protease prostate-specific antigen (PSA) to liberate the active cytotoxin L-leucyl-doxorubicin. Primary cultures of PC-82 human prostate cancer cells secreted high levels of enzymatically active PSA (i.e., 70 ± 5 ng of enzymatically active PSA/106 cells/24 h), whereas LNCaP human prostate cancer ells produced lower levels of enzymatically active PSA (i.e., 2.3 ± 1 ng/106 cells/24 h). LNCaP cells, however, secreted sufficient amounts of enzymatically active PSA to activate the doxorubicin prodrug to a cytotoxic form in vitro. The specificity of the cytotoxic response to the prodrug was demonstrated by the fact that 70 nM of the prodrug killed 50% of the PSA-producing LNCaP cells, whereas doses as high as 1 ♂ had no cytotoxic effect on PSA-nonproducing TSU human prostate cancer cells in vitro.
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| 4. |
- Gao, Ming-Wei, et al.
(author)
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Power predictions for a pilot scale stirred ball mill
- 1996
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In: International Journal of Mineral Processing. - : Elsevier BV. - 0301-7516 .- 1879-3525. ; 44-45, s. 641-652
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Journal article (peer-reviewed)abstract
- The capacity of a stirred ball mill to grind to a certain product size efficiently depends strongly on the power intensity in the milling chamber. The dependence of power intensity on stirrer speed, slurry density, bead density and the amount of dispersant added to the feed was investigated using a 6-litre 11-kW horizontal stirred ball mill fitted with perforated disks as stirrers. Dolomite was ground at a fixed volumetric flow rate, but with slurry densities ranging from 65 to 75 percent by weight, stirrer speeds from 805 to 2253 rpm, bead densities from 2.5 to 5.4 g/ml and the dispersant level from 0.5 to 1.5 percent of dry solids. Three levels of the four variables were used in 27 continuous milling tests, set up as one-third of a 34 factorial design. All factors affected the power draft in a highly significant way. Speed, and to a lesser extent slurry density, were the dominant factors with significant non-linear effects. A six-term model, incorporating all significant effects, predicted the experimental results with an accuracy of about 12%. Increasing the power accelerated size reduction dramatically with only a small change to the energy efficiency of the process.
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| 5. |
- Huang, QW, et al.
(author)
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Effects of inhaled nitric oxide and high-frequency ventilation in rabbits with meconium aspiration
- 1999
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In: Biology of the neonate. - : S. Karger AG. - 0006-3126. ; 76:6, s. 374-382
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Journal article (peer-reviewed)abstract
- Aim: To evaluate effects of inhaled nitric oxide (iNO) in experimental meconium aspiration treated with high-frequency (HFV) or conventional mechanical ventilation (CMV). Ventilated adult rabbits had meconium instilled intratracheally resulting in respiratory failure as evidenced by more than 50% reduction of dynamic lung compliance (Cdyn) and increase in mean oxygenation index (OI) from 1 to 16. The animals were then allocated to 2 groups treated without (control) or with iNO at 20 ppm (NO). In each group the animals were initially ventilated with CMV or HFV mode for 3 h and then in a crossover fashion with HFV or CMV for another 3 h (CMV→HFV, HFV→CMV), respectively. In the first 3 h of treatment, the animals subjected to HFV-CMV in the control, and those with both HFV-CMV and CMV-HFV in the NO group had significantly reduced OI. In the subsequent 3 h, the animals in the control group with CMV-HFV did not improve in OI and those with HFV-CMV had deteriorated. In the NO group with both CMV-HFV and HFV-CMV moderate improvement of OI was observed. Platelet aggregation capability and counts were significantly decreased and bleeding time prolonged in animals receiving iNO treatment. These results suggest that both HFV alone and a combined treatment of iNO with either CMV or HFV are more effective in improving blood oxygenation than that of CMV in this animal model. The influence of iNO on platelet aggregation should be considered.
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| 8. |
- Spies, W, et al.
(author)
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Recombination experiments at CRYRING
- 1998
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In: Hyperfine Interactions. - 0304-3843. ; 114:1-4, s. 237-243
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Journal article (peer-reviewed)abstract
- Recent advances in studies of electron-ion recombination processes at low relative energies with the electron cooler of the heavy-ion storage ring CRYRING are shown. Through the use of an adiabatically expanded electron beam, collisions down to 10(-4) eV relative energies were measured with highly charged ions stored in the ring at around 15 MeV/amu energies. Examples of recombination measurements for bare ions of D+, He2+, N7+, Ne10+ and Si14+ are presented. Further on, results of an experiment measuring laser-induced recombination (LIR) into n = 3 states of deuterium with polarized laser light are shown.
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| 9. |
- Weller, K. R., et al.
(author)
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Media wear in stirred milling
- 1997
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In: Particulate Science and Technology. - : Informa UK Limited. - 0272-6351 .- 1548-0046. ; 15:2, s. 132-
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Journal article (peer-reviewed)abstract
- A method for measuring media wear using a commercially available 0.751 stirred mill is described. Five material groups, namely steel, ceramics, natural materials, glass, and ore pebbles (autogenous grinding) were tested using water alone and a 60% by weight slurry of sulfide ore. Other variables were stirrer speed and media size. The relative wear rates of the different materials and their effects on grinding efficiency are reported. Relative costs of media were taken into account in the assessment. Ottawa sand was the most cost effective medium for wear, and steel shot for grinding efficiency.
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