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- Ghasemi, J., et al.
(author)
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Spectroscopic characterization of thiazole orange-3 DNA interaction
- 2008
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In: Applied Biochemistry and Biotechnology. - : Springer Science and Business Media LLC. - 0273-2289 .- 1559-0291. ; 149:1, s. 9-22
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Journal article (peer-reviewed)abstract
- The interaction of a new derivative of thiazole orange (TO-3) with calf thymus DNA (ctDNA) has been investigated by fluorescence and absorption spectroscopy. When TO-3 binds to ctDNA, absorption bands exhibit significant hypochromicity at low base pair/dye ratio (BP/D ratio), and high BP/D show hyperchromicity with red shift. The spectral changes are attributed to the different species formed between TO-3 and ctDNA in the titration course of the dye molecule with DNA. Multivariate curve resolutions-alternating least squares (MCR-ALS) is applied to the absorption measurements recorded to recover the concentration profiles and the pure spectra of the DNA/TO-3 complexes involved in the process. The binding constant and size of the binding site have been determined spectrophotometrically using the McGhee von Hippel equation. MCR-ALS has been used to reveal the precise concentration profiles of all detectable species formed between ctDNA and TO-3 and their pure spectral profiles. © 2008 Humana Press Inc.
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| 3. |
- Ghasemi, J., et al.
(author)
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A new algorithm for the determination of protolytic constants from spectrophotometric data in multiwavelength mode : Calculations of acidity constants of 4-(2-pyridylazo)resorcinol (PAR) in mixed nonaqueous-water solvents
- 2006
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In: Talanta. - : Elsevier BV. - 0039-9140 .- 1873-3573. ; 68:4, s. 1201-1214
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Journal article (peer-reviewed)abstract
- A new efficient, simple and versatile algorithm is presented for determination of the protolytic constants from spectrophotmetric data in multiwavelength mode based on the combining of hard and soft modeling. The algorithm was checked by determining the acidity constants of a triprotic acid from theoretical and real absorption-pH data. The real spectral data are obtained from photometric titration of different solutions of 4-(2-pyridylazo)resorcinol (PAR) by a standard base solution under an inert atmosphere. The algorithm starts the minimization process using an user supplied number of components and initial guesses of the unknown parameters and refined in a least squares manner. New algorithm is implemented in the new version of DATAN package (version 3.1). The validity of the obtained results was checked by some well known programs such as DATAN 2.1, SPECFIT/32, SQUAD, a modified version of difference spectra and a A-pH curve method. The comparison of the outputs of the DATAN 3.1 with the other programs reveals that there is a very good agreement between the obtained results and mentioned programs.
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- Nategh, Shafigh, 1984-, et al.
(author)
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An Improved Fuzzy Model Based Sensorless Control for Six-Phase Induction Machines
- 2008
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In: 2008 3rd IEEE Conference on Industrial Electronics and Applications, ICIEA 2008. - 9781424417186 ; , s. 1469-1474
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Conference paper (peer-reviewed)abstract
- The presented paper proposes a new scheme for the speed sensorless control of six phase induction motor. This method is based on the current estimation, in which the controlled stator voltage is applied to the induction motor so that the difference between stator currents of the mathematical model and motor can be forced to decay to zero. To make a better dynamic response and reduce the transient time, conventional PI controller has improved using non-linear scale controller. Also fuzzy logic controller has been used to obtain synchronous speed and eliminate steady-state speed error. The performance of the proposed algorithm is verified through simulation.
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