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- Gomila, Margarita, et al.
(author)
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Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water.
- 2007
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In: International journal of systematic and evolutionary microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 57:11, s. 2629-2635
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Journal article (peer-reviewed)abstract
- Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769T, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575T, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class Betaproteobacteria and were related to the genus Pelomonas, with 16S rRNA gene sequence similarities higher than 99% with the only species of the genus, Pelomonas saccharophila and to Pseudomonas sp. DSM 2583. The type strains of Mitsuaria chitosanitabida and Roseateles depolymerans were their closest neighbours (97.9 and 97.3% 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [hoxG (hydrogenase), cbbL/cbbM (Rubisco) and nifH (nitrogenase)] relevant for the metabolism of the genus Pelomonas. DNA-DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of Pelomonas saccharophila obtained from different culture collections (ATCC 15946T, CCUG 32988T, DSM 654T, IAM 14368T and LMG 2256T), as well as M. chitosanitabida IAM 14711T and R. depolymerans CCUG 52219T. Results of DNA-DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the Pelomonas saccharophila type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus Pelomonas, for which the names Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are proposed. The type strains of Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are CCUG 52769T (=CECT 7234T) and CCUG 52575T (=CECT 7233T), respectively.
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| 2. |
- Gomila, Margarita, et al.
(author)
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Description of Roseateles aquatilis sp nov and Roseateles terrae sp nov., in the class Betaproteobacteria, and emended description of the genus Roseateles
- 2008
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In: International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 58, s. 6-11
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Journal article (peer-reviewed)abstract
- Three strains of aerobic Gram-negative bacilli, two isolated from industrial water and freshwater (strains CCUG 48205T and CCUG 52220) and the third from soil (strain CCUG 52222T), were analysed phenotypically and genotypically to clarify their taxonomic classification. 16S rRNA gene sequence analysis revealed that the three strains were located on the same phylogenetic branch, closely related to Roseateles depolymerans, the only recognized species in the genus. DNA–DNA hybridization studies, analyses of fatty acid contents, and physiological and biochemical tests supported the proposal of two novel species, Roseateles aquatilis sp. nov. (type strain, CCUG 48205T=CECT 7248T) and Roseateles terrae sp. nov. (type strain, CCUG 52222T=CECT 7247T).
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| 3. |
- Gomila, Margarita, et al.
(author)
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Molecular Techniques for the Identification of Clinical and Environmental Achromobacter strains
- 2009
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In: FEMS 2009 - 3rd Congress of European Microbiologists.
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Conference paper (other academic/artistic)abstract
- Background: The genus Achromobacter (βetaproteobacteria) comprises six species isolated from different sources, most from clinical samples. The ability to detect and correctly identify A. xylosoxidans and related Gram-negative, non-fermenting species is essential for patients with cystic fibrosis, as well as in nosocomial infections. Traditional phenotyping is not adequate for reliable, definitive identifications of Achromobacter species. Furthermore, sequence analyses of 16S rRNA gene sequences are also not able insure accurate identification of Achromobacter species, due to the limited resolution of the rRNA genes (greater than 98.5% to each other). Objectives: The objective of this study has been to establish a genotypic, multi-locus sequence analysis (MLSA), analysis for the reliable, definitive and cost-effective identification of Achromobacter species, and to apply the molecular tools for typing and identification of clinical isolates. Methods: 1) Selection of phenotypically well-described strains of Achromobacter species isolated from different sources (clinical and environmental). 2) Development of a MLSA strategy based on sequences of 16S rRNA, inter-genic spacer regions (IGS1), DNA gyrase subunit B (gyrB), and RNA polymerase subunit B (rpoB). 3) Comparison and correlation of MLSA data with other methodologies: DNA-DNA hybridisation; fingerprinting analysis; cellular fatty acids analysis; and MALDI-TOF mass spectrometry analyses. Results: Individual phylogenetic trees, as well as combined datasets were compared. The combined analysis of the MLSA data provided differentiation at the intra- and the interspecies levels and reliable identifications of isolates of Achromobacter spp. Conclusions: The MLSA methodology described allows for the rapid and reliable identification of the Achromobacter species, distinguished from related Gram-negative non-fermenting organisms, and is also useful for strain differentiation and typing in molecular epidemiological studies.
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