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- Chung, C M, et al.
(author)
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Amplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
- 2005
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In: Molecular Carcinogenesis. - : Wiley. - 1098-2744 .- 0899-1987. ; 43:3, s. 165-174
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Journal article (peer-reviewed)abstract
- Immortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled Pi artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis.
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- Yang, Bo, et al.
(author)
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Fair resource allocation using bargaining over OFDMA relay networks
- 2009
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In: Proceedings of the 48th IEEE Conference on Decision and Control, 2009 held jointly with the 2009 28th Chinese Control Conference. CDC/CCC. - : IEEE. - 9781424438716 ; , s. 585-590
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Conference paper (peer-reviewed)abstract
- This paper studies fair resource allocation schemes for orthogonal frequency-division multiple-access (OFDMA) with the decode-and-forward relaying strategy. To satisfy the heterogenous rate requirement of each user while considering the fairness and efficiency as performance indices, the bargaining theory is applied to allocate resource at a relay node to multiple source nodes. Motivated by the fact that each source's achievable rate on individual sub-carrier is limited by decodability constraint, we apply the Kalai-Smorodinsky bargaining (KSB) theory to allocate resource at the relay fairly. KSB ensures that all sources incur the same surplus rate penalty relative to the maximal surplus rate. To improve system efficiency, the Nash bargaining (NB) problem is formulated to maximize NB solution fairness criterion by exploiting multiuser diversity. A simple yet efficient algorithm is derived to assign sub-carrier and power at the relay for multiple users. Simulation results demonstrate that both KSB-based solution and NB solution can improve total rates while maintaining fairness among sources.
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