| 1. |
- Malmqvist, K. G., et al.
(author)
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PIXE and proton microprobe advances at the Lund Institute of Technology
- 1989
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In: Nuclear Inst. and Methods in Physics Research, B. - 0168-583X. ; 40-41:PART 1, s. 685-689
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Journal article (peer-reviewed)abstract
- A review of recent advances in high-energy ion beam analysis at the Lund Institute of Technology is presented. A nonvacuum specimen chamber allows chemical speciation using a combination of ion beam analysis and controlled heating. The development of a new versatile scanning proton microbeam based on a new dedicated accelerator, an achromatic triplet lens and an advanced specimen chamber is outlined together with the performance of a microVAX-II/VMEbus-based data acquisition system.
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| 2. |
- Bock, K, et al.
(author)
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Specificity of binding of a strain of uropathogenic Escherichia coli to Gal alpha 1----4Gal-containing glycosphingolipids.
- 1985
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In: The Journal of biological chemistry. - 0021-9258. ; 260:14, s. 8545-51
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Journal article (peer-reviewed)abstract
- A strain of Escherichia coli originally isolated from urine of a patient with acute pyelonephritis was studied in detail for binding to glycosphingolipids. Bacteria labeled metabolically with [14C]glucose were layered over a glycolipid chromatogram and bound bacteria were detected by autoradiography. The detection was down to a few ng of glycolipid (pmol level) under these assay conditions. At a test level of 500 ng all glycolipids (more than a dozen molecular species analyzed) with Gal alpha 1----4Gal as an internal or terminal part bound the bacteria strongly while glycolipids known to lack this sequence were negative. Conformational analysis using hard sphere calculations including the exo-anomeric effect showed a bend in the saccharide chain at this disaccharide with a largely hydrophobic surface of the convex side, probably being part of the binding epitope. Mixtures of glycolipids isolated from a human ureter scraping and from urinary sediments bound bacteria in the 2- to 7-sugar interval. Thus, this infectious strain of E. coli recognizes glycolipids being present in epithelial cells lining the urinary tract.
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| 3. |
- Hansson, H A, et al.
(author)
-
Evidence indicating trophic importance of IGF-I in regenerating peripheral nerves
- 1986
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In: Acta Physiologica Scandinavica. - : Wiley. - 0001-6772 .- 1365-201X. ; 126:4, s. 14-609
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Journal article (peer-reviewed)abstract
- The mechanisms influencing regeneration of peripheral nerves are incompletely known, but growth factors are supposed to play a key role. In the present study, we demonstrate, with the aid of immunohistochemical methods, that somatomedin C (Sm-C/insulin-like growth factor I/IGF-I) rapidly increased from low to high concentrations, reaching peak values in 2 weeks, in regenerating sciatic nerves of adult rats. In addition, IGF-I was demonstrated extracellularly, never observed in the control nerves. Reactive Schwann cells appeared to be the major source for IGF-synthesis. Higher concentrations were seen in tubulated nerves as compared to sutured ones. It is proposed that IGF-I exerts important growth supporting effects on regenerating peripheral nerves.
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| 4. |
- Finne, J, et al.
(author)
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Novel polyfucosylated N-linked glycopeptides with blood group A, H, X, and Y determinants from human small intestinal epithelial cells.
- 1989
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In: The Journal of biological chemistry. - 0021-9258. ; 264:10, s. 5720-35
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Journal article (peer-reviewed)abstract
- A novel type of N-linked glycopeptides representing a major part of the glycans in human small intestinal epithelial cells from blood group A and O individuals were isolated by gel filtrations and affinity chromatography on concanavalin A-Sepharose and Bandeiraea simplicifolia lectin I-Sepharose. Sugar composition, methylation analysis, 1H NMR spectroscopy of the underivatized glycopeptides and FAB-mass spectrometry and electron impact-mass spectrometry of the permethylated glycopeptides indicated a tri- and tetra-antennary structure containing an intersecting N-acetylglucosamine and an alpha (1----6)-linked fucose residue in the core unit for the majority of the glycans. In contrast to most glycopeptides of other sources, the intestinal glycopeptides were devoid of sialic acid, but contained 6-7 residues of fucose. The outer branches contained the following structures: Fuc alpha 1-2Gal beta 1-3GleNAc beta 1- (H type 1) Fuc alpha 1-2Gal beta 1-4GleNAc beta 1- (H type 2) Gal beta 1-4 (Fuc alpha 1-3)GlcNAc beta 1- (X) Fuc alpha 1-2Gal beta 1-4(Fuc alpha 1-3)GleNAc beta 1- (Y) GalNAc alpha 1-3(Fuc alpha 1-2)Gal beta 1-3GleNAc beta 1- (A type 1) GalNAc alpha 1-3(Fuc alpha 1-2)Gal beta 1-4GleNAc beta 1- (monofucosyl A type 2) GalNAc alpha 1-3(Fuc alpha 1-2)Gal beta 1-4 (Fuc alpha 1-3)GlcNAc beta 1- (trifucosyl A type 2) The blood group determinant structures were mainly of type 2, whereas glycolipids from the same cells contained mainly type 1 determinants. The polyfucosylated glycans represent a novel type of blood group active glycopeptides. The unique properties of the small intestinal glycopeptides as compared with glycopeptides of other tissue sources may be correlated with the specialized functional properties of the small intestinal epithelial cells.
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| 5. |
- Hansson, H. C., et al.
(author)
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PIXE in complex analytical systems for atmospheric chemistry
- 1987
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In: Nuclear Inst. and Methods in Physics Research, B. - 0168-583X. ; 22:1-3, s. 235-240
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Journal article (peer-reviewed)abstract
- A schematic mechanistic box model presenting processes in atmospheric chemical cycles is used to present the main research objectives. From this the main use of PIXE within atmospheric chemistry is discussed. An analytic system giving molecular composition is necessary to fully understand and quantify the chemical and physical pathways described in the model for different compounds. Combining PIXE with complementary techniques can enhance knowledge of molecular composition. Therefore, techniques which are complementary to PIXE that give such important parameters as mass, soot, and major ions are discussed. Difficulties in efficiently combining and using the discussed analytical techniques in practice are recognized. The sampling substrates and sampling methods used are discussed and evaluated from this point of view. The use of thin Al foil as substrate and an electrostatic precipitator for aerosol sampling is suggested as one solution. The importance of developing methods for use of this analytical system of the different phases present in the atmosphere is stressed.
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| 6. |
- Lundborg, G, et al.
(author)
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Intraneural edema following exposure to vibration
- 1987
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In: Scandinavian Journal of Work, Environment and Health. - 0355-3140. ; 13:4, s. 9-326
-
Journal article (peer-reviewed)abstract
- Peripheral neuropathy represents a well-known complication from long-term exposure to vibration. In the present study an experimental model is presented with the purpose of analyzing the formation of intraneural edema following vibration exposure. Vibration (82 Hz, peak-to-peak amplitude 0.21 mm) was induced in the hind limb of rats by the use of vibrating electric motors during 4 h/d for 5 d. Tracer techniques (with albumin Evans blue and horseradish peroxidase) were used to study the permeability of intraneural microvessels after the vibration exposure on day 5. It was found that the vibration trauma in this model induced epineurial edema in the sciatic nerve. It is hypothesized that the formation of intraneural edema may be an important pathophysiological factor in the occurrence of vibration-induced neuropathy.
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