| 1. |
- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Hudson, Thomas J., et al.
(author)
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International network of cancer genome projects
- 2010
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 464:7291, s. 993-998
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Journal article (peer-reviewed)abstract
- The International Cancer Genome Consortium (ICGC) was launched to coordinate large-scale cancer genome studies in tumours from 50 different cancer types and/or subtypes that are of clinical and societal importance across the globe. Systematic studies of more than 25,000 cancer genomes at the genomic, epigenomic and transcriptomic levels will reveal the repertoire of oncogenic mutations, uncover traces of the mutagenic influences, define clinically relevant subtypes for prognosis and therapeutic management, and enable the development of new cancer therapies.
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| 3. |
- Wood, Marilee
(author)
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Interconnections : Glass beads and trade in southern and eastern Africa and the Indian Ocean - 7th to 16th centuries AD
- 2012
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Doctoral thesis (other academic/artistic)abstract
- Glass beads comprise the most frequently found evidence of trade between southern Africa and the greater Indian Oceanbetween the 7th and 16th centuries AD. In this thesis beads recovered from southern African archaeological sites are organized into series, based on morphology and chemical composition determined by LA-ICP-MS analysis. The results are used to interpret the trade patterns and partners that linked eastern Africa to the rest of the Indian Ocean world, as well as interconnections between southern Africa andEast Africa. Comprehensive reports on bead assemblages from several archaeological sites are presented, including: Mapungubwe, K2 and Schroda in the Shashe-Limpopo Basin; Chibuene in southern Mozambique; Hlamba Mlonga in eastern Zimbabwe; Sibudu Cave in KwaZulu-Natal, Kaole Ruins in Tanzania and Mahilaka in northwest Madagascar. The conclusions reached show that trade relationships and socio-political development in the south were different from those on the East Coast and that changes in bead series in the south demonstrate it was fully integrated into the cycles of the Eurasian and African world-system.
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| 4. |
- Esterhuysen, Amanda, et al.
(author)
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Archaeology and education
- 2013
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In: <em>The Oxford</em> <em>Handbook of African Archaeology</em>. - Oxford : Oxford University Press. ; , s. 239-252
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Book chapter (peer-reviewed)
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| 5. |
- Kirchhof, Paulus, et al.
(author)
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Comprehensive risk reduction in patients with atrial fibrillation : emerging diagnostic and therapeutic options - a report from the 3rd Atrial Fibrillation Competence NETwork/European Heart Rhythm Association consensus conference
- 2012
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In: Europace. - : Oxford University Press (OUP). - 1099-5129 .- 1532-2092. ; 14:1, s. 8-27
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Research review (peer-reviewed)abstract
- While management of atrial fibrillation (AF) patients is improved by guideline-conform application of anticoagulant therapy, rate control, rhythm control, and therapy of accompanying heart disease, the morbidity and mortality associated with AF remain unacceptably high. This paper describes the proceedings of the 3rd Atrial Fibrillation NETwork (AFNET)/European Heart Rhythm Association (EHRA) consensus conference that convened over 60 scientists and representatives from industry to jointly discuss emerging therapeutic and diagnostic improvements to achieve better management of AF patients. The paper covers four chapters: (i) risk factors and risk markers for AF; (ii) pathophysiological classification of AF; (iii) relevance of monitored AF duration for AF-related outcomes; and (iv) perspectives and needs for implementing better antithrombotic therapy. Relevant published literature for each section is covered, and suggestions for the improvement of management in each area are put forward. Combined, the propositions formulate a perspective to implement comprehensive management in AF.
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| 6. |
- Lane, Andrew M.
(author)
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Do Emotion Regulation Intentions and StrategiesDiffer Between Situations?
- 2014
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In: Current Advances in Psychology. - : American V-King Scientific Publishing. ; 1:1, s. 26-32
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Journal article (other academic/artistic)abstract
- The present study examined relationships between actual and desired emotional states, meta-beliefs concerning the utility of distinct emotions, and emotion regulation strategies used by individuals in a sport situation as well as an emotion-eliciting situation from a different aspect of their lives. Participants (N = 924) reported their emotions, meta-beliefs for optimal emotional states, and their use of emotion regulation strategies across two broad categories of situations: Before sports competition, and a situation from daily life. Results indicated that prior to competition, high activation emotions such as anger, anxiety and excitement were preferred. In terms of strategy use, analyses revealed greater intention to use of strategies intended to increase pleasant and unpleasant emotions were associated with daily life. In conclusion, results indicated that meta-beliefs for optimal emotional states, and strategies used to regulate emotions vary between situations. We suggest that the ability to regulate emotions in a flexible manner to suit the specific dynamics of various situations is proposed to be helpful in the pursuit of personally meaningful goals and that training of a variety of emotion regulation skills could be beneficial.
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| 7. |
- Lane, Andrew M., et al.
(author)
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Emotions and emotion regulation among novice military parachutists
- 2012
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In: Military Psychology. - : Informa UK Limited. - 0899-5605 .- 1532-7876. ; 24:3, s. 331-345
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Journal article (peer-reviewed)abstract
- Soldiers (N = 95) reported emotions and emotion regulation strategies experienced in their first parachute jump and other challenging situations. Results indicated an emotional profile characterized by feeling anxious, energetic, and happy before parachuting and playing sport. However, this pattern was not similar to the emotional responses experienced at work or in life in general. Participants reported greater use of strategies to increase unpleasant emotions an hour before parachuting than in other situations. Findings suggest that developing training protocols to increase the flexibility and versatility of emotion regulation skills might enhance the preparation of novice soldiers for military duties.
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| 10. |
- Lane, Paul, 1957-, et al.
(author)
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Introducing African archaeology
- 2013
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In: <em>The Oxford</em> <em>Handbook of African Archaeology</em>. - Oxford : Oxford University Press. ; , s. 3-11
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Book chapter (other academic/artistic)
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