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- Larsson, Håkan, 1967-, et al.
(author)
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Välvillighet på de privilegierades villkor
- 2009
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In: Pedagogiska Magasinet. - Stockholm. - 1401-3320. ; :4
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Journal article (pop. science, debate, etc.)abstract
- Texten beskriver i populärvetenskaplig form resultatet av en läroboksgranskning genomförd på uppdrag av Skolverket 2006. I granskningen undersöktes hur sexuell läggning framställdet i ett antal läroböcker, hur heteronormen tog sig uttryck samt vilken läsare läroböckerna förutsatte.
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- Nutu, Magdalena, 1967, et al.
(author)
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Distribution and hormonal regulation of membrane progesterone receptors beta and gamma in ciliated epithelial cells of mouse and human fallopian tubes.
- 2009
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In: Reproductive biology and endocrinology : RB&E. - : Springer Science and Business Media LLC. - 1477-7827. ; 7:89
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Journal article (peer-reviewed)abstract
- BACKGROUND: The controlled beating of cilia of the fallopian tube plays an important role in facilitating the meeting of gametes and subsequently transporting the fertilized egg to its implantation site. Rapid effects of progesterone on ciliary beat frequency have been reported in the fallopian tubes of cows, but the identity of the receptors mediating this non-genomic action of progesterone is not known. We recently identified a member of the non-genomic membrane progesterone receptor family, mPR gamma, as a candidate for mediating these actions of progesterone. Here, we investigated the possible presence of a related receptor, mPR beta, in the fallopian tubes of mice and women as well as the possible hormonal regulation of mPR beta and gamma. METHODS: Western blot and immunohistochemistry with specific antibodies were used to characterize the expression and cellular localization of the mPRs in mouse and human tissues. Taqman (Quantitative Polymerase Chain Reaction) assays were used to quantify mRNA levels in the fallopian tubes of two different mouse models after injections with different hormones and specific antagonists. RESULTS: In the fallopian tubes of both mouse and human, the expression of mPR beta and mPR gamma proteins was exclusively found in the ciliated cells. Whereas mPR beta was found on the cilia, mPR gamma was localized at the base of the same ciliated cells, as previously reported. In gonadotropin-primed mice, both mPRs genes were down-regulated after an injection with progesterone. Treatment with estradiol rapidly down-regulated the level of mPR beta mRNA and protein in immature mice. The mPR gamma protein was down-regulated around the time of ovulation in cycling women, similar to the regulation observed in mice stimulated to ovulate via gonadotropin injections. CONCLUSION: Our findings show the presence and hormonal regulation of two distinct mPRs associated with the cilia of the fallopian tubes in both mice and women. It is hypothesized that these receptors are involved in the control of ciliary movement and, thus, gamete transport in the fallopian tubes of mammals.
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- Nutu, Magdalena, 1967, et al.
(author)
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Membrane progesterone receptor gamma: tissue distribution and expression in ciliated cells in the fallopian tube.
- 2007
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In: Molecular reproduction and development. - : Wiley. - 1040-452X .- 1098-2795. ; 74:7, s. 843-50
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Journal article (peer-reviewed)abstract
- Non-genomic, rapid actions of steroids have long been known, suggesting the possible presence of non-classical steroid receptors. A membrane receptor for progestins (mPR) was recently described in the spotted seatrout, and transcripts of three related receptors (alpha, beta, and gamma) were subsequently identified in other species including human and mouse. To begin exploring the roles of mPRgamma in mammals, we have generated an antibody against this receptor. The specificity of the antibody was demonstrated by both overexpression and RNA interference experiments. Using the antibody, we show that mPRgamma is expressed in female mouse reproductive tissues such as ovary and fallopian tube, and also in the lung and liver of both sexes. Immunohistochemical studies revealed that mPRgamma is associated with the apical membrane of ciliated cells facing the lumen of the fallopian tube. The presence of mPRgamma in ciliated cells of the fallopian tube was also demonstrated in human samples. Rapid effects of progesterone on ciliary beat frequency in the fallopian tube have recently been reported. Together, this suggests a common role for mPRgamma in the regulation of ciliary activity in the fallopian tube and thus gamete transport in mammals. The presence of mPRgamma in lung and liver of mice suggests that the receptor mediates the actions of progesterone outside the reproductive tract as well.
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- Nutu, Magdalena, 1967, et al.
(author)
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Progesterone membrane receptors - regulation in the ovary of the rat.
- 2005
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In: Proceedings in: Steroids. Poster presentation at the FASEB conference on "Steroid Hormone Receptors: Integration of Plasma Membrane and Nuclear-Initiated Signaling in the Hormone Action", July 31 - Aug 5, 2004, Tucson, Arizona, USA. ; 70, s. 478-9
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Conference paper (peer-reviewed)
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