| 1. |
- Guerra, Lina, et al.
(author)
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Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage
- 2010
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In: PLOS ONE. - : Public Library Science. - 1932-6203. ; 5:1
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Journal article (peer-reviewed)abstract
- BACKGROUND: The MYC protein controls cellular functions such as differentiation, proliferation, and apoptosis. In response to genotoxic agents, cells overexpressing MYC undergo apoptosis. However, the MYC-regulated effectors acting upstream of the mitochondrial apoptotic pathway are still unknown.PRINCIPAL FINDINGS: In this study, we demonstrate that expression of Myc is required to activate the Ataxia telangiectasia mutated (ATM)-dependent DNA damage checkpoint responses in rat cell lines exposed to ionizing radiation (IR) or the bacterial cytolethal distending toxin (CDT). Phosphorylation of the ATM kinase and its downstream effectors, such as histone H2AX, were impaired in the myc null cell line HO15.19, compared to the myc positive TGR-1 and HOmyc3 cells. Nuclear foci formation of the Nijmegen Breakage Syndrome (Nbs) 1 protein, essential for efficient ATM activation, was also reduced in absence of myc. Knock down of the endogenous levels of MYC by siRNA in the human cell line HCT116 resulted in decreased ATM and CHK2 phosphorylation in response to irradiation. Conversely, cell death induced by UV irradiation, known to activate the ATR-dependent checkpoint, was similar in all the cell lines, independently of the myc status.CONCLUSION: These data demonstrate that MYC contributes to the activation of the ATM-dependent checkpoint responses, leading to cell death in response to specific genotoxic stimuli.
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| 2. |
- Gunnarsdottir, Anna, et al.
(author)
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Quality of Life in Adults Operated On for Hirschsprung Disease in Childhood.
- 2010
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In: Journal of Pediatric Gastroenterology and Nutrition - Jpgn. - 1536-4801. ; 51, s. 160-166
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Journal article (peer-reviewed)abstract
- OBJECTIVES:: The aim of this study is to explore the long-term quality of life (QoL) in adults after surgery for Hirschsprung disease in childhood. PATIENTS AND METHODS:: Altogether 51 patients were operated for HD at our center during the period 1969 to 1989. In 2007, the validated instruments for assessing QoL, the SF-36 health survey and the Gastrointestinal Quality of Life Index (GIQLI), were sent to the 47 patients still alive at the time of the study. A retrospective review of the patient records was also done. RESULTS:: Forty-two patients returned both questionnaires (89% response rate) at the median age of 28.5 (range 18-45) years. The median clinical follow-up time was 5.7 years (range 5 months-23 years). At the last clinical control, 4 (9%) patients had a terminal enterostomy, 12 (29%) had soiling, 5 (12%) had constipation, and 2 (5%) experienced recurrent enterocolitis. In contrast to males, the subscores for females were lower for general health and mental health than for an age- and sex-matched general population (P < 0.05). Patients having aganglionosis to the right colon had lower GIQLI scores than those with aganglionosis to the left colon (P < 0.05). In multivariate linear regression analysis, female sex was the only factor associated with lower GIQLI score (P < 0.05). CONCLUSIONS:: The long-term QoL of adults operated for Hirschsprung disease in their youth is satisfactory. Female scores were lower for general and mental health, compared with the matched control group. The study indicates that the longer the aganglionic segment, the greater its impact on QoL in later life.
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| 3. |
- Laurenius, Anna, et al.
(author)
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Changes in eating behaviour and meal pattern following Roux-en-Y gastric bypass.
- 2012
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In: International journal of obesity. - : Springer Science and Business Media LLC. - 1476-5497 .- 0307-0565. ; 36:3, s. 348-355
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Journal article (peer-reviewed)abstract
- Background:Little is known about eating behaviour and meal pattern subsequent to Roux-en-Y gastric bypass (RYGB), knowledge important for the nutritional care process. The objective of the study was to obtain basic information of how meal size, eating rate, meal frequency and eating behaviour change upon the RYGB surgery.Materials and methods:Voluntary chosen meal size and eating rate were measured in a longitudinal, within subject, cohort study of 43 patients, 31 women and 12 men, age 42.6 (s.d. 9.7) years, body mass index (BMI) 44.5 (4.9) kgm(-2). Thirty-one non-obese subjects, 37.8 (13.6) years, BMI 23.7 (2.7) kgm(-2) served as a reference group. All subjects completed a meal pattern questionnaire and the Three-Factor Eating Questionnaire (TFEQ-R21).Results:Six weeks postoperatively meal size was 42% of the preoperative meal size, (P<0.001). After 1 and 2 years, meal size increased but was still lower than preoperative size 57% (P<0.001) and 66% (P<0.001), respectively. Mean meal duration was constant before and after surgery. Mean eating rate measured as amount consumed food per minute was 45% of preoperative eating rate 6 weeks postoperatively (P<0.001). After 1 and 2 years, eating rate increased to 65% (P<0.001) and 72% (P<0.001), respectively, of preoperative rate. Number of meals per day increased from 4.9 (95% confidence interval, 4.4,5.4) preoperatively to 6 weeks: 5.2 (4.9,5.6), (not significant), 1 year 5.8 (5.5,6.1), (P=0.003), and 2 years 5.4 (5.1,5.7), (not significant). Emotional and uncontrolled eating were significantly decreased postoperatively, (both P<0.001 at all-time points), while cognitive restraint was only transiently increased 6 weeks postoperatively (P=0.011).Conclusions:Subsequent to RYGB, patients display markedly changed eating behaviour and meal patterns, which may lead to sustained weight loss.International Journal of Obesity advance online publication, 29 November 2011; doi:10.1038/ijo.2011.217.
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| 4. |
- Sjövie, Helen, et al.
(author)
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Postoperative gastrostomy site leakage correlated to the dimension of the gastrostomy button in children
- 2010
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In: Gastroenterology Insights. - : MDPI AG. - 2036-7422 .- 2036-7414. ; Vol 2:No 2, s. 34-36
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Journal article (peer-reviewed)abstract
- Abstract The laparoscopic placement of gastrostomy buttons for feeding tubes is the preferred method of gastrostomy in children with feeding requirements. This intervention often leads to some minor postoperative problems, including gastrostomy site leakage. The aim of our study was to test the hypothesis that the postoperative leakage after a video-assisted gastrostomy is correlated to the dimension of the gastrostomy button used during the oper - ation. Included in the study were 164 children with nutritional problems who consecutively underwent surgery; they had a video-assisted gastrostomy operation. In the first 87 children, a 14 French (Fr) gastrostomy button was used and in the last 77, a 12 Fr button was used. After the operation, the children were followed up prospectively and all complications were documented according to the study protocol. Our study revealed a significant correlation between the dimension of the gastrostomy button and the postoperative leakage at the gastrostomy site. The rate of leakage at the gastrostomy site was 37% in the children who had 14 Fr gastrostomy buttons compared to 18% (P=0.038) in the children who had 12 Fr gastrostomy buttons, during the first six months postoperatively. These results suggest that postoperative gastrostomy site leakage was significantly higher in children who had 14 Fr gastrostomy buttons than in those with 12 Fr buttons. To avoid this complication, 12 Fr gastro stomy buttons should be used.
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