| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Lundberg, C, et al.
(author)
-
Inflammatory reaction in an experimental model of open wounds in the rat. The role of polymorphonuclear leukocytes.
- 1984
-
In: Laboratory Investigation. - 0023-6837 .- 1530-0307. ; 50:6, s. 726-32
-
Journal article (peer-reviewed)abstract
- The accumulation of polymorphonuclear leukocytes (PMNLs) and the concentration of prostaglandin E2 in full thickness open skin wounds, created in the back of the rat, were studied. The quantity of PMNLs in the granulation tissue, assessed by analysis of wound exudate myeloperoxidase activity, increased from 0.45 units/ml on day 1 after wounding to 0.8 units/ml on day 2 and then remained constant throughout the 5 days of observation. The prostaglandin E2 concentration in wound exudate was measured by radioimmunoassay increased progressively from 70 ng/ml on day 1 to 290 ng/ml on day 5. The lack of correlation between these two variables indicates that PMNLs were not the major source of prostaglandin E2. To study the relation between PMNL accumulation, blood flow, and albumin extravasation in the granulation tissue, rats were treated with an antineutrophil serum. This resulted in an 83% decrease in circulating PMNLs and a 61% decrease in granulation tissue myeloperoxidase activity on the 5th day, as compared with rats treated with normal rabbit serum. No relationship was observed between myeloperoxidase activity and blood flow (r = 0.37) or between myeloperoxidase activity and albumin extravasation (r = -0.34) when absolute values were compared. However, when interindividual variation in the absolute levels of the variable measured was eliminated, by calculating in each rat a left to right wound ratio, good correlations (r = 0.81 and r = 0.65, respectively) were found. It is suggested that blood flow and albumin extravasation in the granulation tissue are influenced by local PMNL accumulation.
|
|
| 8. |
- Lundberg, C, et al.
(author)
-
The inflammatory reaction in an experimental model of open wounds in the rat. The effect of arachidonic acid metabolites.
- 1984
-
In: European Journal of Pharmacology. - 0014-2999 .- 1879-0712. ; 97:3-4, s. 229-38
-
Journal article (peer-reviewed)abstract
- The study concerned the effect of arachidonic acid metabolites on the inflammatory reaction in granulation tissue of open wounds in rats. Metabolites or inhibitors were applied in a wound chamber attached to circular, open, full-thickness skin wounds 5 days post-wounding. The adjacent wound served as control. Blood flow, albumin extravasation and accumulation of polymorphonuclear leucocytes (PMNLs) were measured in the granulation tissue. Prostaglandin E2 (PGE2 5.7 microM) increased blood flow and albumin extravasation by 95 and 16%, respectively, without affecting PMNLs. Leukotriene B4 (LTB4 2.7 microM) increased PMNL accumulation by 142% without altering albumin extravasation. Indomethacin (28 microM, repeatedly) did not affect blood flow or albumin extravasation, but increased PMNL accumulation by 21%. Methylprednisolone (3.3 mM, repeatedly) reduced blood flow and albumin extravasation by 29 and 31%, respectively, without influencing PMNLs. The granulation tissue obviously responds to exogenous PGE2 and LTB4. Endogenous arachidonic acid metabolites seem to play only a minor role in the inflammatory process in this model.
|
|
| 9. |
- Lundberg, C, et al.
(author)
-
The inflammatory reaction in healing wounds : the role of polymorphonuclear leucocytes.
- 1984
-
In: International journal on tissue reactions. - 0250-0868. ; 6:6, s. 477-83
-
Journal article (peer-reviewed)abstract
- The inflammatory process in granulation tissue in full-thickness skin wounds was studied and the role of polymorphonuclear leucocytes (PMNLs) in this process evaluated in an experimental model in the rat. The number of PMNLs in the wound, assessed by determination of the PMNL-specific enzyme myeloperoxidase (MPO) activity in wound exudate, increased from 0.45 U/ml on day 1 after wounding to 0.8 U/ml on day 2, and then remained constant throughout the five days of observation. The concentration of prostaglandin E2 (PGE2) in wound exudate increased progressively from 70 ng/ml on day 1 to 290 ng/ml on day 5. The lack of correlation between these two variables indicated that PMNLs were not the major source of PGE2. Blood flow and albumin extravasation in the granulation tissue were measured, and the relation between these two variables and PMNL accumulation was studied. Rats were rendered neutropenic with an antineutrophil serum, resulting in an 83% decrease in circulating PMNLs and a 61% decrease in granulation tissue MPO activity on day 5, as compared with rats treated with normal rabbit serum. These reductions did not, however, affect either blood flow or albumin extravasation, and no correlation was observed; but when inter-individual variations in the absolute levels of the variables measured were eliminated by calculating in each rat a left-to-right wound ratio, PMNL accumulation correlated well to both blood flow (R = 0.81) and albumin extravasation (R = 0.65). It is suggested that blood flow and albumin extravasation in the granulation tissue are influenced by local PMNL accumulation and, further, that the inflammatory response varies considerably between one animal and another.
|
|
| 10. |
- Lundberg, C, et al.
(author)
-
The role of histamine and serotonin in the inflammatory reaction in an experimental model of open wounds in the rat.
- 1984
-
In: Scandinavian journal of plastic and reconstructive surgery. - 0036-5556. ; 18:2, s. 175-80
-
Journal article (peer-reviewed)abstract
- The role of histamine and serotonin in the inflammatory reaction in the granulation tissue of open wounds in the rat was studied. The model involved plastic chambers attached to the edges of two open circular full-thickness skin wounds. Five days post-wounding, agonists or antagonists were applied in one of the two chambers, the adjacent wound serving as control. Thereafter blood flow and albumin extravasation were measured. Application of histamine (100 microM) caused an increase in granulation tissue blood flow by 36%, but left albumin extravasation unaffected. Treatment with mepyramine (H1 antagonist, 20 microM), cimetidine (H2 antagonist, 20 microM) or methysergide (serotonin antagonist, 20 microM) did not influence the level of either blood flow or albumin extravasation. It is suggested that endogeneous histamine and serotonin play a minor role in the inflammatory process in the granulation tissue of this model of healing wounds.
|
|
