| 1. |
- Decou, Raphaël, et al.
(författare)
-
Analysis of lignin composition and distribution using fluorescence laser confocal microspectroscopy
- 2017. - 1
-
Ingår i: Xylem. - New York : Humana Press. - 9781493967209 - 9781493967223 ; , s. 233-247
-
Bokkapitel (refereegranskat)abstract
- Lignin is a polyphenolic polymer specifically accumulating in the cell walls of xylem cells in higher vascular plants. Far from being homogeneous, the lignification of xylem cell walls varies in deposition site, quantity, composition and macromolecular conformation depending on the cell wall compartment, cell type, cell developmental stage and plant species. Here, we describe how confocal microspectroscopy methods using lignin autofluorescence can be used to evaluate the relative lignin amounts, its spatial distribution and composition at the cellular and sub-cellular levels in both isolated cells and histological cross-sections of plant tissues.
|
|
| 2. |
- Menard, Delphine, et al.
(författare)
-
Establishment and utilization of habituated cell suspension cultures for hormone-inducible xylogenesis
- 2017. - 1
-
Ingår i: Xylem. - New York : Humana Press. - 9781493967209 - 9781493967223 ; , s. 37-57
-
Bokkapitel (refereegranskat)abstract
- The development of inducible cell differentiation in suspension cultures led to multiple breakthroughs. It enabled the understanding of the chronology, duration, regulation and interdependency of the multiple events leading to fully functional specialized cells. The most studied cell differentiation in plants using inducible suspension cultures is the formation of tracheary elements (TEs) - the hydro-mineral sap conducting cells. Several in vitro systems established in different plant species have been developed to trigger TE formation on-demand. Here, we describe the establishment, harvesting and analysis of Arabidopsis thaliana stable habituated cell lines inducible by hormones to differentiate into TEs on-demand. Moreover, we explain the means to monitor and modify the chronology, duration and regulation of the progression of TE formation.
|
|
