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Träfflista för sökning "WFRF:(Martin Davila P.) srt2:(2012-2014)"

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1.	Eyre, S, et al. (author) High-density genetic mapping identifies new susceptibility loci for rheumatoid arthritis 2012 In: Nature genetics. - : Springer Science and Business Media LLC. - 1546-1718 .- 1061-4036. ; 44:12, s. 1336-1340 Journal article (peer-reviewed)
2.	Balao da Silva, C. M., et al. (author) Effect of Overnight Staining on the Quality of Flow Cytometric Sorted Stallion Sperm: Comparison with Tradtitional Protocols 2014 In: Reproduction in domestic animals. - : Wiley. - 0936-6768 .- 1439-0531. ; 49:6, s. 1021-1027 Journal article (peer-reviewed)abstract Flow cytometry is considered the only reliable method for the separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter is highly efficient, allowing the production of foals of the desired sex. However, to achieve acceptable pregnancy rates the currently used protocol requires working with fresh semen obtained close to, or at, the sorting facility. An alternative protocol was tested during two consecutive breeding seasons. Fresh stallion semen was cooled for 20 h, during which staining with Hoechst 33342 took place. On the following day, this sample was flow sorted and compared with spermatozoa from the same ejaculate that had been sexed on the previous day. All sperm parameters evaluated remained unchanged when fresh sorted and refrigerated sorted semen were compared. Pre-sorting storage at 5 degrees C did not alter sperm velocities nor kinetics, viability or membrane permeability, production of reactive oxygen species, mitochondrial membrane potential or DNA fragmentation index of the sorted sample. The findings open for the possibility of using semen from stallions housed far from the sorting facilities. Processed and stained sperm could be shipped refrigerated on the previous day, sorted and inseminated on the next day.

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Type of publication: journal article (2)

Type of content: peer-reviewed (2)

Author/Editor: Martin, J. (1); KLARESKOG, L (1); Lee, A. (1); Raychaudhuri, S (1); Martin, P. (1); Rodriguez-Martinez, ... (1); show more...; Morrell, Jane (1); Wijmenga, C (1); Padyukov, L (1); Gregersen, PK (1); Worthington, J (1); Amos, CI (1); Gonzalez-Gay, MA (1); Alfredsson, L (1); Symmons, D. (1); Onengut-Gumuscu, S (1); Deloukas, P. (1); Hu, XL (1); Huizinga, TWJ (1); Rantapää-Dahlqvist, ... (1); Plenge, RM (1); Zhernakova, A (1); Hunt, SE (1); Edkins, S (1); Langford, C (1); Franke, L (1); Westra, HJ (1); Stahl, E (1); Khor, CC (1); Heng, KK (1); Davila, S (1); Ortega-Ferrusola, C (1); Balao da Silva, C. M ... (1); Plaza Davila, M. (1); Martin-Munoz, P. (1); Morrell, J. M. (1); Pena, F. J. (1); Concannon, P (1); Rich, SS (1); Barton, A. (1); Eyre, S (1); de Bakker, PIW (1); Toes, REM (1); Rodriguez-Rodriguez, ... (1); Ärlestig, Lisbeth (1); Diogo, D (1); Trynka, G (1); Bowes, J (1); Viatte, S (1); McAllister, K (1); show less...

University: Umeå University (1); Linköping University (1); Karolinska Institutet (1); Swedish University of Agricultural Sciences (1)

Language: English (2)

Research subject (UKÄ/SCB): Medical and Health Sciences (2); Agricultural Sciences (1)

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