| 1. |
- Cameron, AD, et al.
(author)
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Crystal structure of human glyoxalase .1. Evidence for gene duplication and 3D domain swapping
- 1997
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In: EMBO JOURNAL. - : OXFORD UNIV PRESS. - 0261-4189. ; 16:12, s. 3386-3395
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Journal article (other academic/artistic)abstract
- The zinc metalloenzyme glyoxalase I catalyses the glutathione-dependent inactivation of toxic methylglyoxal. The structure of the dimeric human enzyme in complex with S-benzyl-glutathione has been determined by multiple isomorphous replacement (MIR) and r
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| 3. |
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| 4. |
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| 5. |
- Mannervik, B, et al.
(author)
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An evolutionary approach to the design of glutathione-linked enzymes
- 1998
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In: CHEMICO-BIOLOGICAL INTERACTIONS. - : ELSEVIER SCI IRELAND LTD. - 0009-2797. ; 112, s. 15-21
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Journal article (other academic/artistic)abstract
- Studies of protein structure provide information about principles of protein design that have come into play in natural evolution. This information can be exploited in the redesign of enzymes for novel functions. The glutathione-binding domain of glutathi
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| 7. |
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| 8. |
- Kim, Y B, et al.
(author)
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Atomic force microscopy of rubbed polyimide aligning films for liquid crystal displays
- 1995
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In: 15th International Liquid Crystal ConferenceBUDAPEST, HUNGARY, JUL 03-08, 1994. ; , s. 89-98
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Conference paper (peer-reviewed)abstract
- The surface of polyimide (PI) films for aligning liquid crystals were studied by means of atomic force microscopy (AFM). The surface of the unrubbed PI films consisted of polymer clusters of different sizes which are randomly distributed over the film area. After rubbing, however, these polymer clusters formed long chains along the rubbing direction. The cluster chains were separated with periodicity about 100 nm for weak rubbing strength. Deeper grooves are also present, separated with periodicity about 2 mu m that could be related to the microstructure of the fibres from the rubbing cloth. On increasing the rubbing strength further on, the cluster chains coalesce into wider ones. The rubbed PI films show optical retardation, which was increased rapidly with increased the rubbing strength and reached a constant value of about 1.4nm.
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| 9. |
- Ljunghusen, O, et al.
(author)
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Phenotypic alterations in circulating monocytes induced by open heart surgery using heparinized and nonheparinized cardiopulmonary bypass systems.
- 1997
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In: Artificial Organs. - : Wiley. - 0160-564X .- 1525-1594. ; 21, s. 1091-
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Journal article (peer-reviewed)abstract
- In this study of 31 patients with coronary bypass surgery, we used flow cytometry to compare heparin-coated and noncoated cardiopulmonary bypass systems on leukocyte activation. We found significant differences between the groups during bypass, with activation of the complement system, measured as elevated levels of C3a desArg, upregulation of granulocyte beta2 integrin (CD11b), and a loss of circulating monocytes when noncoated systems were used. In both groups an early increase in the monocyte cell surface CD62L expression was obvious while the percentage of human leukocyte antigen (HLA)-DR positive monocytes did not alter. The morning after the operation, leukocytosis was present, together with a highly significant reduction in the monocyte expression of CD11b and HLA-DR, indicating the recruitment to the peripheral blood of cells with altered phenotypes. This alteration in phenotype on potent inflammatory cells may be one part of the impaired function of the immunological system reported after major surgery.
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| 10. |
- Noresson, B, et al.
(author)
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Effects of capacity on the preconcentration of trace metals and matrix elimination by an iminodiacetate chelating adsorbent
- 1998
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In: Talanta. - 0039-9140. ; 46:5, s. 1051-1063
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Journal article (peer-reviewed)abstract
- Low capacity adsorbents, based on iminodiacetic acid and a crosslinked agarose, Novarose(TM), have been synthesised for the enrichment of metal ions. Test ions Cu2+ with high, and Cd2+ with a moderate affinity for the sorbent were selected. The study includes the effect of specific capacity and matrix composition on the rate of uptake and recovery of these ions. Using a column packed with 0.25 ml of a sorbent and a sample volume of 100 ml, Cu2+ is quantitatively accumulated from all of the matrices studied even at a capacity of 6 mu mol ml(-1) of adsorbent and a flow rate as high as 100 ml min(-1). The enrichment of Cd2+ is affected by the matrix and for quantitative recovery the flow rate and capacity must be properly adjusted. In the presence of 0.01 M Ca2+, a specific capacity of 45 mu mol ml(-1) is needed for quantitative retainment and a flow rate of 100 ml min(-1), whereas a capacity of 10 mu mol ml(-1) suffices at 10 ml min(-1). The advantage of low specific capacity on the removal of matrix elements has been demonstrated. A method based on the determination of conditional stability constants of the metal sorbent complex is suggested for predicting the sorbent capacity needed to acquire quantitative recovery and optimal matrix elimination.
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